Clonal Species Trichoderma parareesei sp. nov. Likely Resembles the Ancestor of the Cellulase Producer Hypocrea jecorina/T. reesei
Clonal Species Trichoderma parareesei sp. nov. Likely Resembles the Ancestor of the Cellulase Producer Hypocrea jecorina/T. reesei
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DOI：
10.1128/aem.01184-10
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Druzhinina, Irina S.
中科院分区：
文献类型：
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作者：
Atanasova, Lea;Jaklitsch, Walter M.;Druzhinina, Irina S.
我们以前曾报道过，著名的工业酶生产者里氏木霉（远形霉霉）（霉霉霉；霉霉霉，子囊菌，Dikarya）有一个基因分离的同居姐妹种，没有有性繁殖，由大多数以前归因于霉霉/T的变形菌株组成。reesei。在本文中，我们提出了这一新物种T. parareesei的正式分类描述，并补充了多变量表型分析和分子进化研究。对RNA聚合酶B亚基II （rpb2）和GII18几丁质酶（chi18-5）编码片段等相对保守位点的系统发育分析表明，副螺旋体在遗传上是不变的，可能与饲养螺旋体的祖先相似。这一点，以及至少有一个配对型的T. parareesei基因已经被发现与猪锥虫/T的序列相比发生了本质上的改变。Reesei指出，分化的发生可能是由于两个物种的假设祖先交配系统的功能受损。相比之下，我们发现有性繁殖和相应的更多的多态H. jecorina/T。Reesei本质上是进化而来的。在7种温度方案下进行的表型微阵列分析支持了我们之前的推测，即T. parareesei具有相对较高的机会性潜力，这可能确保了该物种在热带森林等古老和可持续的环境中生存。
We have previously reported that the prominent industrial enzyme producer Trichoderma reesei (teleomorph Hypocrea jecorina; Hypocreales, Ascomycota, Dikarya) has a genetically isolated, sympatric sister species devoid of sexual reproduction and which is constituted by the majority of anamorphic strains previously attributed to H. jecorina/T. reesei. In this paper we present the formal taxonomic description of this new species, T. parareesei, complemented by multivariate phenotype profiling and molecular evolutionary examination. A phylogenetic analysis of relatively conserved loci, such as coding fragments of the RNA polymerase B subunit II (rpb2) and GII18 chitinase (chi18-5), showed that T. parareesei is genetically invariable and likely resembles the ancestor which gave raise to H. jecorina. This and the fact that at least one mating type gene of T. parareesei has previously been found to be essentially altered compared to the sequence of H. jecorina/T. reesei indicate that divergence probably occurred due to the impaired functionality of the mating system in the hypothetical ancestor of both species. In contrast, we show that the sexually reproducing and correspondingly more polymorphic H. jecorina/T. reesei is essentially evolutionarily derived. Phenotype microarray analyses performed at seven temperature regimens support our previous speculations that T. parareesei possesses a relatively high opportunistic potential, which probably ensured the survival of this species in ancient and sustainable environment such as tropical forests.