D-alanyl-lipoteichoic acid in Lactobacillus casei: secretion of vesicles in response to benzylpenicillin.
D-alanyl-lipoteichoic acid in Lactobacillus casei: secretion of vesicles in response to benzylpenicillin.
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干酪乳杆菌中的 D-丙氨酰脂磷壁酸：响应青霉素的囊泡分泌。
DOI：
10.1099/00221287-138-5-849
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Neuhaus,FC
中科院分区：
文献类型：
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作者：
Pollack,JH;Ntamere,AS;Neuhaus,FC
从在青霉素或d -环丝氨酸存在下生长的干酪乳杆菌atcc 7469中分离出含有脂磷胆酸（LTA）的囊泡。这些细胞壁抗生素使LTA和脂质分泌率提高6.7倍，而氯霉素则抑制它们的释放。这些囊泡的形成来自外周和间隔壁区域，似乎不是细菌溶解的结果。LTA和脂质组成与细胞质膜相似，而蛋白质组成不同。这些囊泡的大小在20 ~ 40 nm之间，LTA的长度在5 ~ 50个磷酸甘油残基之间。这些囊泡的分离为研究脂磷胆酸的d -丙烯酰化提供了一个潜在的体外受体系统。
Vesicles containing lipoteichoic acid (LTA) have been isolated fromLactobacillus caseiATCC 7469 grown in the presence of either benzylpenicillin or D-cycloserine. These cell wall antibiotics enhanced the rate of LTA and lipid secretion 6.7 times, whereas chloramphenicol inhibited their release. The formation of these vesicles from peripheral and septal wall regions did not appear to be the result of bacteriolysis. The vesicle composition of LTA and lipid was similar to that of the cytoplasmic membrane whereas the protein composition was dissimilar. The size of these vesicles ranged from 20 to 40 nm and the length of LTA ranged from 5 to 50 glycerol phosphate residues. The isolation of these vesicles provides a potentialin vitroacceptor system for studying the D-alanylation of lipoteichoic acid.