Dynamic reprogramming of H3K9me3 at hominoid-specific retrotransposons during human preimplantation development
Dynamic reprogramming of H3K9me3 at hominoid-specific retrotransposons during human preimplantation development
复制标题
人类植入前发育过程中人科动物特异性逆转录转座子上 H3K9me3 的动态重编程
DOI：
10.1016/j.stem.2022.06.006
复制
发表时间：
2022-07-07
期刊：
影响因子：
23.9
通讯作者：
Wang, Jichang
中科院分区：
文献类型：
--
作者：
Yu, Hanwen;Chen, Manqi;Wang, Jichang
h3k9me3依赖性异染色质的重编程是早期发育所必需的。然而，H3K9me3是如何参与人类早期发育的，在很大程度上仍不清楚。在这里，我们解决了H3K9me3在人类胚胎植入前发育过程中的时间格局，以及它对多种人类特异性反转录转座子的调控。在8细胞阶段，H3K9me3在类人猿特异性反转录转座子sin - vntr - alu （SVA）上重编程，促进了某些启动子和SVA衍生的增强子之间的相互作用，促进了合子基因组的激活。在滋养外胚层中，新生H3K9me3结构域阻止多能转录因子与类人类特异性反转录转座子衍生的内细胞团（ICM）特异性基因调控元件结合。与人类多能干细胞相比，ICM中SVA元素的H3K9me3重建与DNA修复基因的高转录相关。我们的数据表明，类人猿特异性反转录转座子上h3k9me3依赖性异染色质的物种特异性重组在人类早期发育过程中发挥了重要作用，揭示了哺乳动物早期发育的表观遗传调控是如何进化的。
Reprogramming of H3K9me3-dependent heterochromatin is required for early development. How H3K9me3 is involved in early human development remains, however, largely unclear. Here, we resolve the temporal landscape of H3K9me3 during human preimplantation development and its regulation for diverse homi-noid-specific retrotransposons. At the 8-cell stage, H3K9me3 reprogramming at hominoid-specific retrotransposons termed SINE-VNTR-Alu (SVA) facilitates interaction between certain promoters and SVA-derived enhancers, promoting the zygotic genome activation. In trophectoderm, de novo H3K9me3 domains prevent pluripotent transcription factors from binding to hominoid-specific retrotransposons-derived regulatory elements for inner cell mass (ICM)-specific genes. H3K9me3 re-establishment at SVA el-ements in the ICM is associated with higher transcription of DNA repair genes, when compared with naive human pluripotent stem cells. Our data demonstrate that species-specific reorganization of H3K9me3-dependent heterochromatin at hominoid-specific retrotransposons plays important roles during early human development, shedding light on how the epigenetic regulation for early development has evolved in mammals.