INHIBITION OF ESCHERICHIA-COLI H+-ATPASE BY VENTURICIDIN, OLIGOMYCIN AND OSSAMYCIN
INHIBITION OF ESCHERICHIA-COLI H+-ATPASE BY VENTURICIDIN, OLIGOMYCIN AND OSSAMYCIN
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DOI：
10.1016/0005-2728(85)90254-3
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
SENIOR, AE
中科院分区：
文献类型：
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作者：
PERLIN, DS;LATCHNEY, LR;SENIOR, AE
研究了抗生素文丘里菌素、寡霉素和奥沙霉素作为大肠杆菌 H+-ATP 酶的潜在抑制剂。研究发现，文丘里菌素强烈抑制 ATP 驱动的质子转运和 ATP 水解，而寡霉素则微弱抑制这些功能。文丘里菌素和寡霉素对 H+-ATP 酶的抑制与 F0 介导的质子转运的抑制相关。发现两种抑制剂都会干扰二环己基[14C]碳二亚胺和 F0 亚基 c（uncE 蛋白）之间的共价反应。奥沙霉素对大肠杆菌F0或F1无直接抑制作用；相反，它被发现可以将 ATP 水解与质子传输分开。
The antibiotics venturicidin, oligomycin and ossamycin were investigated as potential inhibitors of the E. coli H+-ATPase. It was found that venturicidin strongly inhibited ATP-driven proton transport and ATP hydrolysis, while oligomycin weakly inhibited these functions. Inhibition of the H+-ATPase by venturicidin and oligomycin was correlated with inhibition of F0-mediate proton transport. Both inhibitors were found to interfere with the covalent reaction between dicyclohexyl[14C]carbodiimide and the F0 subunit c (uncE protein). Ossamycin had no direct inhibitory effect on E. coli F0 or F1; rather, it was found to uncouple ATP hydrolysis from proton transport.