Hydrostatic forces limit swelling of rat ventricular myocardium.
Hydrostatic forces limit swelling of rat ventricular myocardium.
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静水力限制大鼠心室心肌的肿胀。
DOI：
10.1152/ajpheart.1981.241.5.h740
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Abelmann,WH
中科院分区：
文献类型：
--
作者：
Pine,MB;Brooks,WW;Nosta,JJ;Abelmann,WH
为了研究当细胞膜和离子泵不能防止肿胀时的心室细胞容积调节，将大鼠心室切片在改良的Krebs-Henseleit溶液中孵育，其中1）钾取代钠，离子取代离子;或2）氯化钠减少以将渗透压降低至228，171或114 mosM。测量心室水、[3 H]菊粉和[3 H]甘露醇空间、钾、钠、氯和蛋白质含量以及静息跨膜电位。心室细胞体积的增加小于30%，钾替代和极稀的媒体（114 mosM），在相同的治疗肾皮质切片的增加超过100%。在钾替代溶液中，心室细胞在孵育期间获得的液体相对于洗浴介质是高渗的。在稀溶液（171和114 mosM），心室，细胞和细胞外渗透压平衡后，才发生了大量的细胞离子损失。这些发现支持心室细胞肿胀存在机械限制，这可能是由心室心肌中存在的独特间质胶原网络引起的。
To study ventricular cellular volume regulation when cell membranes and ion pumps cannot prevent swelling, rat ventricular sections were incubated in modified Krebs-Henseleit solutions in which 1) potassium was substituted for sodium, ion for ion; or 2) sodium chloride was reduced to decrease osmolarity to 228, 171, or 114 mosM. Ventricular water, [3H]inulin and [3H]mannitol spaces, potassium, sodium, chloride, and protein contents, and resting transmembrane potentials were measured. Increases in ventricular cellular volume were less than 30% in potassium-substituted and extremely dilute media (114 mosM), in contrast to increases of over 100% in identically treated renal cortical slices. In potassium-substituted solution, the fluid gained by ventricular cells during incubation was hypertonic with respect to the bathing medium. In dilute solution (171 and 114 mosM), ventricular, cellular, and extracellular osmolarities equilibrated only after substantial losses of cellular ions had occurred. These findings support the existence of mechanical limitations to ventricular cellular swelling, which may be caused by a unique network of interstitial collagen present in ventricular myocardium.