Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater.
Bioaugmentation of membrane bioreactor with Achromobacter denitrificans strain PR1 for enhanced sulfamethoxazole removal in wastewater.
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DOI：
10.1016/j.scitotenv.2018.08.100
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发表时间：
2019-01
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通讯作者：
P. Nguyen;A. Silva;A. Reis;O. Nunes;A. Rodrigues;J. Rodrigues;V. Cardoso;M. Benoliel;M. Reis;A. Oehmen;G. Carvalho
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作者：
P. Nguyen;A. Silva;A. Reis;O. Nunes;A. Rodrigues;J. Rodrigues;V. Cardoso;M. Benoliel;M. Reis;A. Oehmen;G. Carvalho
脱氮嗜铬杆菌PR1是一株具有较高磺胺甲恶唑(SMX)降解率的特殊降解途径菌株，在好氧条件下运行于实验室规模的膜生物反应器(MBR)中，用于处理含SMX的实际生活污水。考察了不同水力停留时间(HRT)对SMX去除效果的影响，发现HRTs与反应时间和负荷有关。在生物强化和非生物强化活性污泥(AS)中，初级底物的可用性对于SMX的共代谢都是重要的。由于底物的限制，高水力停留时间(24 h)导致低的食物微生物比(F/M)和低的SMX去除。水力停留时间从24 h减少到12 h、6 h和4 h时，有机物和氨氮等主要基质的有效性逐渐增加，从而提高了SMX的去除效率和降解率，更有利于高负荷污水处理工艺。在接种到MBR后，尽管观察到丰度显著下降，生物强化菌株在反应器中的最长持续时间为31 天。与对照MBR相比，生物强化MBR对SMX的去除效果更好，尤其是在SMX冲击负荷下。这项研究的结果表明，需要定期重新接种一段时间后，以保持对目标化合物的去除效率。
Achromobacter denitrificansstrain PR1, previously found to harbour specific degradation pathways with high sulfamethoxazole (SMX) degradation rates, was bioaugmented into laboratory-scale membrane bioreactors (MBRs) operated under aerobic conditions to treat SMX-containing real domestic wastewater. Different hydraulic retention times (HRTs), which is related to reaction time and loading rates, were considered and found to affect the SMX removal efficiency. The availability of primary substrates was important in both bioaugmented and non-bioaugmented activated sludge (AS) for cometabolism of SMX. High HRT (24 h) resulted in low food to microorganism ratio (F/M) and low SMX removal, due to substrate limitation. Decrease in HRT from 24 h to 12 h, 6 h and finally 4 h led to gradual increases in primary substrates availability, e.g. organic compounds and ammonia, resulted in increased SMX removal efficiency and degradation rate, and is more favorable for high-rate wastewater treatment processes. After inoculation into the MBRs, the bioaugmentation strain was sustained in the reactor for a maximum of 31 days even though a significant decrease in abundance was observed. The bioaugmented MBRs showed enhanced SMX removal, especially under SMX shock loads compared to the control MBRs. The results of this study indicate that re-inoculation is required regularly after a period of time to maintain the removal efficiency of the target compound.