Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor
Pathogen- and NaCl-induced expression of the SCaM-4 promoter is mediated in part by a GT-1 box that interacts with a GT-1-like transcription factor
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DOI：
10.1104/pp.104.041442
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发表时间：
2004-08-01
期刊：
影响因子：
7.4
通讯作者：
Cho, MJ
中科院分区：
文献类型：
--
作者：
Park, HC;Kim, ML;Cho, MJ
在高等真核生物中，钙（2+）结合蛋白钙调蛋白介导细胞钙（2+）信号以响应广泛的刺激。植物表达许多CaM同工异构体。一种大豆（Glycine max） CaM亚型SCaM-4的转录在病原体或NaCl胁迫下30分钟内被显著诱导。为了表征该基因的顺式作用元件，我们分离了该基因约2kb的启动子序列。对启动子的缺失分析显示，应激源诱导SCaM-4表达需要位于-858和-728核苷酸位置之间的130 bp区域。该区域的六聚体DNA序列GAAAAA （GT-1顺式元件）被鉴定为诱导SCaM-4基因的核心顺式作用元件。在体外和酵母选择系统中，GT-1顺式元件与拟南芥GT-1样转录因子AtGT-3b相互作用。AtGT-3b在病原体和NaCl处理后30分钟内也能快速诱导转录。这些结果表明，GT-1顺式元件和GT-1样转录因子之间的相互作用在大豆和拟南芥中病原体和盐诱导的SCaM-4基因表达中起作用。
The Ca(2+)-binding protein calmodulin mediates cellular Ca(2+) signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter sequence of the gene. Deletion analysis of the promoter revealed that a 130-bp region located between nucleotide positions -858 and -728 is required for the stressors to induce expression of SCaM-4. A hexameric DNA sequence within this region, GAAAAA (GT-1 cis-element), was identified as a core cis-acting element for the induction of the SCaM-4 gene. The GT-1 cis-element interacts with an Arabidopsis GT-1-like transcription factor, AtGT-3b, in vitro and in a yeast selection system. Transcription of AtGT-3b is also rapidly induced within 30 min after pathogen and NaCl treatment. These results suggest that an interaction between a GT-1 cis-element and a GT-1-like transcription factor plays a role in pathogen- and salt-induced SCaM-4 gene expression in both soybean and Arabidopsis.