Expansion of the global RNA virome reveals diverse clades of bacteriophages
Expansion of the global RNA virome reveals diverse clades of bacteriophages
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DOI：
10.1016/j.cell.2022.08.023
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发表时间：
2022-10-13
期刊：
影响因子：
64.5
通讯作者：
Gophna, Uri
中科院分区：
文献类型：
--
作者：
Neri, Uri;Wolf, Yuri I.;Gophna, Uri
高通量RNA测序为探索地球RNA病毒体提供了广阔的机会。挖掘5,150个不同的转录本，发现250万个RNA病毒重叠群。对>；330,000个依赖RNA的RNA聚合酶(RdRPs)的分析表明，这种扩大对应于已知RNA病毒多样性的5倍增加。基因含量分析揭示了以前在RNA病毒中没有发现的多个蛋白质结构域，并与病毒与宿主的相互作用有关。扩展的RDRP系统发育支持这五个已建立的门的单系性，并揭示了两个假定的附加噬菌体门和许多假定的附加纲和目。急剧扩大的Lenarviricota门，由细菌和相关的欧盟核病毒组成，现在占rna病毒体的三分之一。对CRISPR间隔区匹配和溶菌蛋白的鉴定表明，以前与真核生物相关的微小核糖核酸病毒和部分病毒亚群感染原核宿主。
High-throughput RNA sequencing offers broad opportunities to explore the Earth RNA virome. Mining 5,150 diverse metatranscriptomes uncovered >2.5 million RNA virus contigs. Analysis of >330,000 RNA-dependent RNA polymerases (RdRPs) shows that this expansion corresponds to a 5-fold increase of the known RNA vi-rus diversity. Gene content analysis revealed multiple protein domains previously not found in RNA viruses and implicated in virus-host interactions. Extended RdRP phylogeny supports the monophyly of the five es-tablished phyla and reveals two putative additional bacteriophage phyla and numerous putative additional classes and orders. The dramatically expanded phylum Lenarviricota, consisting of bacterial and related eu-karyotic viruses, now accounts for a third of the RNA virome. Identification of CRISPR spacer matches and bacteriolytic proteins suggests that subsets of picobirnaviruses and partitiviruses, previously associated with eukaryotes, infect prokaryotic hosts.