16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams.
16S rRNA assessment of the influence of shading on early-successional biofilms in experimental streams.
复制标题
16S rRNA 评估遮蔽对实验流中早期连续生物膜的影响。
DOI：
10.1093/femsec/fiv129
复制
发表时间：
2015
影响因子：
4.2
通讯作者：
Lehmann K
中科院分区：
文献类型：
--
作者：
Lehmann K
营养物水平升高可能导致生物膜过度生长，但减少营养物污染通常具有挑战性。因此，人们有兴趣制定控制营养丰富河流中生物膜生长的措施，这些措施可以作为直接减少营养负荷的补充。河流遮荫是减轻水华的一种选择，但很少有研究通过实验检验遮荫和未遮荫条件下生长的生物膜群落的差异。我们利用泰晤士河（英国）内的原位生态系统研究了生物膜群落的组装和多样性。通过 16S 扩增子（覆盖 V6/V7 区域的 400 bp 长度）的 454 次测序来调查生物膜组成。结果证实了阳光对于生物膜群落组装的重要性；一种资源被相对较少的优势类群利用，导致其多样性明显低于阴影群落。无遮荫和遮荫处理之间的这些差异要么是由于资源利用的差异，要么是由于作为细菌栖息地的硅藻结构的丧失。我们在阴影社区中观察到更多的共现模式和网络交互。这进一步支持了以下提议：增加河流遮荫有助于减轻河流常量营养素污染的影响。
Elevated nutrient levels can lead to excessive biofilm growth, but reducing nutrient pollution is often challenging. There is therefore interest in developing control measures for biofilm growth in nutrient-rich rivers that could act as complement to direct reductions in nutrient load. Shading of rivers is one option that can mitigate blooms, but few studies have experimentally examined the differences in biofilm communities grown under shaded and unshaded conditions. We investigated the assembly and diversity of biofilm communities usingin situmesocosms within the River Thames (UK). Biofilm composition was surveyed by 454 sequencing of 16S amplicons (∼400 bp length covering regions V6/V7). The results confirm the importance of sunlight for biofilm community assembly; a resource that was utilized by a relatively small number of dominant taxa, leading to significantly less diversity than in shaded communities. These differences between unshaded and shaded treatments were either because of differences in resource utilization or loss of diatom-structures as habitats for bacteria. We observed more co-occurrence patterns and network interactions in the shaded communities. This lends further support to the proposal that increased river shading can help mitigate the effects from macronutrient pollution in rivers.