Platelet rich fibrin matrix effects on skeletal muscle lesions: an experimental study.
Platelet rich fibrin matrix effects on skeletal muscle lesions: an experimental study.
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富含血小板的纤维蛋白基质对骨骼肌病变的影响：一项实验研究。
DOI：
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发表时间：
2012
影响因子：
3.2
通讯作者：
M. Mattioli
中科院分区：
文献类型：
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作者：
A. Gigante;M. Del Torto;S. Manzotti;M. Cianforlini;A. Busilacchi;P. A. Davidson;F. Greco;M. Mattioli
尽管肌肉损伤非常常见，但关于其有效治疗的科学数据很少。生长因子(GFS)可能在加速肌肉修复过程中起作用，目前可用的将其输送到病变部位的策略是使用自体富含血小板血浆(PRP)。本研究的重点是利用富含血小板的纤维蛋白基质(PRFM)作为GFS的来源。在Wistar大鼠背最长肌上造成双侧肌肉损伤。病变一侧用PRFM填充，而对侧不处理(对照)。分别于术后第5、10、40、60天处死动物。组织学、免疫组织化学和组织形态计量学分析评估肌肉再生、新生血管、纤维化和炎症。化生区、钙化和异位骨化的存在也被评估。与对照组相比，经PRFM处理的肌肉表现出更好的肌肉再生、新生血管的增加和轻微的纤维化减轻。在炎症方面没有检测到差异。未见化生、骨化和异位钙化。这项初步的形态实验研究表明，使用PRFM可以促进肌肉再生和长期血管形成。由于自体血液制品是安全的，PRFM在肌肉损伤的临床治疗中可能是一种有用和方便的产品。
Even though muscle injuries are very common, few scientific data on their effective treatment exist. Growth Factors (GFs) may have a role in accelerating muscle repair processes and a currently available strategy for their delivery into the lesion site is the use of autologous platelet-rich plasma (PRP). The present study is focused on the use of Platelet Rich Fibrin Matrix (PRFM), as a source of GFs. Bilateral muscular lesions were created on the longissimus dorsi muscle of Wistar rats. One side of the lesion was filled with a PRFM while the contralateral was left untreated (controls). Animals were sacrificed at 5, 10, 40 and 60 days from surgery. Histological, immunohistochemical and histomorphometric analyses were performed to evaluate muscle regeneration, neovascularization, fibrosis and inflammation. The presence of metaplasia zones, calcifications and heterotopic ossification were also assessed. PRFM treated muscles exhibited an improved muscular regeneration, an increase in neovascularization, and a slight reduction of fibrosis compared with controls. No differences were detected for inflammation. Metaplasia, ossification and heterotopic calcification were not detected. This preliminary morphological experimental study shows that PRFM use can improve muscle regeneration and long-term vascularization. Since autologous blood products are safe, PRFM may be a useful and handy product in clinical treatment of muscle injuries.