Age-dependent differences in the strength and persistence of psychostimulant-induced conditioned activity in rats: effects of a single environment-cocaine pairing.
Age-dependent differences in the strength and persistence of psychostimulant-induced conditioned activity in rats: effects of a single environment-cocaine pairing.
复制标题
DOI：
10.1097/fbp.0000000000000085
复制
发表时间：
2014-10
影响因子：
1.6
通讯作者：
Mohd-Yusof A
中科院分区：
文献类型：
--
作者：
McDougall SA;Pipkin JA;Der-Ghazarian T;Cortez AM;Gutierrez A;Lee RJ;Carbajal S;Mohd-Yusof A
本研究的目的是确定可卡因诱导的条件活动在年轻和成年大鼠的强度和持久性。一个试验协议已被证明是有用的研究个体发育的精神兴奋剂诱导的行为敏化，因此，一个类似的程序被用来检查条件活动。在出生后第19天（PD）或PD 80天，在新的试验室或饲养笼中向大鼠注射生理盐水或可卡因。在各种药物戒断间隔（1-21天）后，向大鼠注射盐水并返回测试室，在那里评估条件活性。在一个单独的实验中，我们检查可卡因诱导的条件反射活动是否是巴甫洛夫条件反射的结果，还是未能适应测试环境。结果表明，成年大鼠表现出强烈的一次试验条件活动，持续至少21天，而年轻的大鼠没有表现出条件运动反应。成年大鼠表现出的条件反射活动并不是由于未能适应可卡因配对的环境。这些结果表明，可卡因配对的上下文刺激差异影响行为，这取决于动物的年龄。成年大鼠提供的数据对人类具有潜在的翻译相关性，因为单一的环境-药物配对导致了行为的长期改变。
The purpose of the present study was to determine the strength and persistence of cocaine-induced conditioned activity in young and adult rats. A one-trial protocol has proven useful for studying the ontogeny of psychostimulant-induced behavioral sensitization; therefore, a similar procedure was used to examine conditioned activity. On postnatal day (PD) 19 or PD 80, rats were injected with saline or cocaine in either a novel test chamber or the home cage. After various drug abstinence intervals (1–21 days), rats were injected with saline and returned to the test chamber, where conditioned activity was assessed. In a separate experiment, we examined whether cocaine-induced conditioned activity was a consequence of Pavlovian conditioning or a failure to habituate to the test environment. The results showed that adult rats exhibited strong one-trial conditioned activity that persisted for at least 21 days, whereas young rats did not show a conditioned locomotor response. The conditioned activity exhibited by adult rats did not result from a failure to habituate to the cocaine-paired environment. These results indicate that cocaine-paired contextual stimuli differentially affect behavior depending on age of the animal. The data provided by adult rats have potential translational relevance for humans because a single environment-drug pairing caused long-term alterations in behavior.