PLAQUE ASSAY AND PRIMARY ISOLATION OF INFLUENZA A VIRUSES IN AN ESTABLISHED LINE OF CANINE KIDNEY-CELLS (MDCK) IN PRESENCE OF TRYPSIN
PLAQUE ASSAY AND PRIMARY ISOLATION OF INFLUENZA A VIRUSES IN AN ESTABLISHED LINE OF CANINE KIDNEY-CELLS (MDCK) IN PRESENCE OF TRYPSIN
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DOI：
10.1007/bf02123572
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发表时间：
1975-01-01
影响因子：
5.4
通讯作者：
FURUYAMA, M
中科院分区：
文献类型：
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作者：
TOBITA, K;SUGIURA, A;FURUYAMA, M
多种甲型流感病毒（包括人、马、猪和禽流感病毒株）在含有胰蛋白酶的覆盖培养基下，在已建立的犬肾细胞系（MDCK）中高效生长，并形成明确的噬斑，无论其先前的传代史如何。噬菌斑形成效率与通过尿囊途径感染受精卵的效率相当。MDCK细胞也已成功地用于从患者的咽洗液中初步分离甲型流感病毒。几个临床标本的平行滴定表明，接种到MDCK细胞，然后在胰蛋白酶的存在下孵育是一个敏感的羊膜接种到受精卵的分离程序。
A wide variety of influenza A viruses, comprising human, equine, porcine, and avian strains, grew productively in an established line of canine kidney cells (MDCK) under an overlay medium containing trypsin, and formed well-defined plaques regardless of their prior passage history. Plaquing efficiency was comparable to the efficiency of infection in fertile eggs via allantoic route. MDCK cells have also been successfully employed for the primary isolation of influenza A virus from throat washings of patients. Parallel titration of several clinical specimens showed that the inoculation into MDCK cells followed by incubation in the presence of trypsin was an isolation procedure as sensitive as the amniotic inoculation into fertile eggs.