Oxidative metabolism in cultured rat astroglia: effects of reducing the glucose concentration in the culture medium and of D-aspartate or potassium stimulation
Oxidative metabolism in cultured rat astroglia: effects of reducing the glucose concentration in the culture medium and of D-aspartate or potassium stimulation
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DOI：
10.1038/sj.jcbfm.9600175
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发表时间：
2006-02-01
影响因子：
6.3
通讯作者：
Suzuki, N
中科院分区：
文献类型：
--
作者：
Abe, T;Takahashi, S;Suzuki, N
培养基中的葡萄糖浓度可能影响培养细胞的能量代谢。星形胶质细胞中葡萄糖的氧化代谢也可能受到影响，因为许多培养基的葡萄糖浓度（25 mmol/ L）高于生理水平（类似于3 mmol/ L）。在本研究中，我们通过测量L-[U-C-14]乳酸或D-[U-C-14]葡萄糖氧化成（CO2）-C-14的速率，评估了降低培养基中葡萄糖浓度对培养的大鼠星形胶质细胞中葡萄糖氧化代谢的影响。还研究了D-天冬氨酸和升高的细胞外K+水平对星形胶质细胞氧化和糖酵解代谢的影响。在含2 mmol/ L葡萄糖的培养基中培养的星形胶质细胞（星形胶质细胞（2））中[C-14]乳酸和[C-14]葡萄糖氧化的速率约为在含22 mmol/ L葡萄糖的培养基中培养的星形胶质细胞（星形胶质细胞（22））中的2倍。D-天冬氨酸（500 μ mol/L）显著增加星形胶质细胞（22）中[C-14]乳酸氧化156%和星形胶质细胞（2）中83%。星形胶质细胞（22）和星形胶质细胞（2）中的D-[U-C-14]葡萄糖氧化也分别增加了94%和76%。相反，细胞外K+浓度升高（7.4 mmol/ L）不影响葡萄糖和乳酸的氧化，虽然它增加了2-脱氧-D-[1-C-14]葡萄糖磷酸化。在生理葡萄糖浓度下生长的星形胶质细胞比在高糖浓度下生长的星形胶质细胞更依赖于葡萄糖的氧化代谢。培养液中的葡萄糖浓度对体外培养的星形胶质细胞的氧化能力有很强的影响。
The glucose concentration in the culture medium may affect the energy metabolism of cultured cells. The oxidative metabolism of glucose in astrocytes might also be affected because the glucose concentration ( 25 mmol/ L) of many culture media is higher than the physiological levels (similar to 3 mmol/ L). In the present study, we assessed the effects of reducing the glucose concentration in the culture medium on the oxidative metabolism of glucose in cultured rat astroglia by measuring the oxidation rates of L-[U-C-14] lactate or D-[U-C-14] glucose to (CO2)-C-14. The effects of D-aspartate and elevated extracellular K+ levels on oxidative and glycolytic metabolism in astroglia were also investigated. The rates of [C-14] lactate and [C-14] glucose oxidation in astroglia cultured in a medium containing 2 mmol/ L of glucose ( astroglia(2)) were approximately twofold of those in astroglia cultured in a medium containing 22 mmol/ L of glucose (astroglia(22)). D- Aspartate (500 mu mol/L) significantly increased [C-14] lactate oxidation by 156% in astroglia(22) and by 83% in astroglia(2). D-[U-C-14] glucose oxidation in astroglia(22) and astroglia(2) was also increased by 94% and 76%, respectively. In contrast, an elevated extracellular K+ concentration (7.4 mmol/ L) did not affect glucose and lactate oxidation, although it increased 2-deoxy-D-[1-C-14] glucose phosphorylation. Astroglia grown in the physiological glucose concentration are more dependent on the oxidative metabolism of glucose than that in high-glucose concentration. Glucose concentration in culture medium has a strong influence on astrocytic oxidative capacity in vitro.