Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses
Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses
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DOI：
10.4161/psb.5.7.11820
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Shigeoka, Shigeru
中科院分区：
文献类型：
--
作者：
Ishikawa, Kazuya;Yoshimura, Kazuya;Shigeoka, Shigeru
在拟南芥水解酶（AtNUDX 1类似于27）中，已发现AtNUDX 6和AtNUDX 7具有ADP-核糖/NADH焦磷酸水解酶活性，有助于保持能量和氧化还原稳态，和/或调节对生物和非生物胁迫的防御反应。有趣的是，AtNUDX 6对免疫应答的调节具有与AtNUDX 7相反的作用。野生型、敲除（KO）-nudx 6和KO-nudx 7植物中ADP-核糖/NADH焦磷酸水解酶活性的比较揭示了在正常条件和氧化胁迫下AtNUDX 7比AtNUDX 6对ADP-核糖和NADH的总焦磷酸水解酶活性的贡献更大，而AtNUDX 6在水杨酸处理下占总NADH焦磷酸水解酶活性的大部分。这些结果支持这样的想法，即ADP-核糖和/或NADH的代谢需要被精细地调节，以准确地调节细胞对生物和非生物胁迫的反应。
Among Arabidopsis Nudix hydrolases (AtNUDX1 similar to 27), AtNUDX6 and AtNUDX7 having ADP-ribose/NADH pyrophosphohydrolase activities have been found to contribute to keeping the energy and redox homeostasis, and/or modulating defense responses against biotic and abiotic stress. Interestingly, AtNUDX6 had an opposite effect to AtNUDX7 on the regulation of immune responses. A comparison of the activities of ADP-ribose/NADH pyrophosphohydrolase among wild-type, knockout (KO)-nudx6, and KO-nudx7 plants revealed AtNUDX7 to contribute more than AtNUDX6 to the total pyrophosphohydrolase activity toward both ADP-ribose and NADH under normal conditions and oxidative stress, while AtNUDX6 accounted for the majority of total NADH pyrophosphohydrolase activity under salicylic acid treatment. These results support the idea that the metabolism of ADP-ribose and/or NADH needs to be finely tuned for accurate regulation of cellular responses to biotic and abiotic stress.