Serum protein profiling of adults and children with Crohn disease.
Serum protein profiling of adults and children with Crohn disease.
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DOI：
10.1097/mpg.0000000000000579
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发表时间：
2015-01
影响因子：
2.9
通讯作者：
Tsangaris GT
中科院分区：
文献类型：
--
作者：
Vaiopoulou A;Gazouli M;Papadopoulou A;Anagnostopoulos AK;Karamanolis G;Theodoropoulos GE;M'Koma A;Tsangaris GT
克罗恩病（CD）和溃疡性结肠炎（UC），统称为炎症性肠病（IBD），是病因不明的慢性免疫炎症性病理。尽管生物标志物在医疗实践中经常使用，但关于IBD的经验证儿科生物标志物的信息相对缺乏。此外，被证明在成人中有效的生物标志物经常被外推到儿科临床环境，而没有考虑许多疾病的发病机制在儿童中明显不同。在本研究中，采用蛋白质组学技术监测成人和儿童CD患者蛋白质表达的差异，以确定一组候选蛋白质生物标志物，可用于提高诊断准确性和促进儿科医疗护理。对12例成人和12例儿童活动性CD患者的男性和女性血清样本进行双向凝胶电泳。通过光密度测定法对两组之间表现出差异表达的蛋白质点进行相对定量后，通过MALDI-TOF-MS对这些点进行进一步表征。结果通过蛋白质印迹分析得到证实。发现CD成人患者的血浆铜蓝蛋白（CLUS）显著过表达，而儿童患者血浆铜蓝蛋白（CERU）和载脂蛋白B-100（APOB）显著过表达，表明这些蛋白的表达可能与这两个亚组患者的CD发作或进展有关。有趣的是，我们发现了成人与儿童CD患者中几种蛋白质的差异表达。毫无疑问，未来需要使用更大的CD患者队列进行实验，以评估我们初步发现的相关性。
Crohn’s disease (CD) and ulcerative colitis (UC), known collectively as inflammatory bowel disease (IBD), are chronic immuno-inflammatory pathologies of unknown etiology. Despite the frequent utilization of biomarkers in medical practice, there is a relative lack of information regarding validated paediatric biomarkers for IBD. Further, biomarkers proved to be efficacious in adults are frequently extrapolated to the paediatric clinical setting without considering that the pathogenesis of many diseases is distinctly different in children. In the current study, proteomics technology was employed in order to monitor differences in protein expression among adult and children CD patients, in order to identify a panel of candidate protein biomarkers that might be used to improve prognostic-diagnostic accuracy and to advance paediatric medical care. Male and female serum samples from 12 adults and 12 children with active CD were subjected to two-dimensional gel electrophoresis. Following the relative quantitation of protein spots exhibiting a differential expression between the two groups by densitometry, the spots were further characterized by MALDI-TOF-MS. Results were confirmed by Western blot analysis. Clusterin (CLUS) was found to be significantly over-expressed in adults with CD, whereas ceruloplasmin (CERU) and apolipoprotein B-100 (APOB) were found to be significantly over-expressed in children indicating that the expression of these proteins might be implicated in the onset or progression of CD in these two sub-groups of patients. Interestingly, we found a differential expression of several proteins in adults versus paediatric CD patients. Undoubtedly, future experiments using a larger cohort of CD patients are needed to evaluate the relevance of our preliminary findings.