Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38-Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway
Ginsenoside Rg1 Inhibits Cell Proliferation and Induces Markers of Cell Senescence in CD34+CD38-Leukemia Stem Cells Derived from KG1α Acute Myeloid Leukemia Cells by Activating the Sirtuin 1 (SIRT1)/Tuberous Sclerosis Complex 2 (TSC2) Signaling Pathway
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人参皂苷 Rg1 通过激活 Sirtuin 1 (SIRT1)/结节性硬化症复合物 2 (TSC2) 信号通路，抑制 KG1a 急性髓系白血病细胞衍生的 CD34 CD38 白血病干细胞中的细胞增殖并诱导细胞衰老标志物
DOI：
10.12659/msm.918207
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发表时间：
2020-02-10
影响因子：
3.1
通讯作者：
Wang, Cui-Li
中科院分区：
文献类型：
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作者：
Tang, Yan-Long;Zhang, Cheng-Gui;Wang, Cui-Li
背景：急性髓系白血病（AML）的临床复发与白血病干细胞（LSC）的治疗反应降低有关。本研究旨在探讨人参衍生物人参皂苷 Rg1 (Rg1) 对源自 KG1 α 人急性髓系白血病细胞的 CD34+CD38- LSC 的影响。 材料/方法：通过细胞分选从 KG1 α 人急性髓系白血病细胞中分离 CD34+CD38- LSCs。 CD34+CD38-KG1αLSC5分为对照组和Rg1组(用Rg1处理)。细胞计数试剂盒-8 (CCK-8) 测定评估 CD34+CD38-KG1 α LSC 的增殖，流式细胞术研究细胞周期。混合集落形成单位 (CFU-Mix) 测定和衰老相关 β-半乳糖苷酶 (SA-β-Gal) 染色评估了细胞衰老。使用蛋白质印迹和定量逆转录聚合酶链反应 (qRT-PCR) 评估沉默调节蛋白 1 (SIRT1) 和结节性硬化症复合物 2 (TSC2) 的表达。结果：CD34+CD38-KG1 α LSC 分离率为 98.72%。与对照组相比，Rg1显着降低CD34+CD38-KG1αLSC5的增殖（p
Background: Clinical relapse in acute myeloid leukemia (AML) is associated with the reduced treatment response of leukemia stem cells (LSCs). This study aimed to investigate the effects of the ginseng derivative, ginsenoside Rg1 (Rg1), on CD34+CD38- LSCs derived from KG1 alpha human acute myeloid leukemia cells.Material/Methods: CD34+CD38- LSCs were isolated from KG1 alpha human acute myeloid leukemia cells by cell sorting. CD34+CD38- KG1 alpha LSC5 were divided into the control group and the Rg1 group (treated with Rg1). The cell counting kit-8 (CCK-8) assay evaluated the proliferation of CD34+CD38- KG1 alpha LSCs and flow cytometry studied the cell cycle. The mixed colony-forming unit (CFU-Mix) assay and staining for senescence-associated beta-galactosidase (SA-beta-Gal) evaluated cell senescence. Expression of sirtuin 1 (SIRT1) and tuberous sclerosis complex 2 (TSC2) were evaluated using Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR).Results: CD34+CD38- KG1 alpha LSCs were isolated at 98.72%. Rg1 significantly reduced the proliferation of CD34+CD38- KG1 alpha LSC5 compared with the control group (p