The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z
The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z
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DOI：
10.1038/ni1166
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发表时间：
2005-03-01
影响因子：
30.5
通讯作者：
Steiner, LA
中科院分区：
文献类型：
--
作者：
Danilova, N;Bussmann, J;Steiner, LA
到目前为止，硬骨鱼中唯一已知的免疫球蛋白重链类别是Mu和Delta。我们在这里确定了一个以前未知的类别，免疫球蛋白Zeta，在斑马鱼和其他硬骨鱼中表达。在斑马鱼重链基因座中，可变(V)基因片段位于两个串联多样性、连接和恒定(DJC)簇的上游，类似于小鼠T细胞受体α(TCRA)和增量(Tcrd)基因座。V基因重排为(DJC)(Zeta)或(DJC)(Mu)，没有开关重排的证据。位于V基因阵列附近的斑马鱼免疫球蛋白Zeta基因(IgZ)和小鼠Tcrd基因在发育早期表达。在成体中，IGZ仅在肾脏和胸腺表达，而这两个器官是硬骨鱼的主要淋巴器官。这一额外的类别增加了免疫球蛋白谱系的复杂性，并提出了关于免疫球蛋白的进化和IIGZ和IIGM差异表达的调节的问题。
The only immunoglobulin heavy-chain classes known so far in teleosts have been mu and delta. We identify here a previously unknown class, immunoglobulin zeta, expressed in zebrafish and other teleosts. In the zebrafish heavy-chain locus, variable (V) gene segments lie upstream of two tandem diversity, joining and constant (DJC) clusters, resembling the mouse T cell receptor alpha (Tcra) and delta (Tcrd) locus. V genes rearrange to (DJC)(zeta) or to (DJC)(mu) without evidence of switch rearrangement. The zebrafish immunoglobulin zeta gene (ighz) and mouse Tcrd, which are proximal to the V gene array, are expressed earlier in development. In adults, ighz was expressed only in kidney and thymus, which are primary lymphoid organs in teleosts. This additional class adds complexity to the immunoglobulin repertoire and raises questions concerning the evolution of immunoglobulins and the regulation of the differential expression of ighz and ighm.