The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae.
The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae.
复制标题
Dcr2p 磷酸酶使酿酒酵母中的 Sic1p 不稳定。
DOI：
10.1016/j.bbrc.2007.07.092
复制
发表时间：
2007
影响因子：
3.1
通讯作者：
Polymenis,Michael
中科院分区：
文献类型：
--
作者：
Pathak,Ritu;Blank,HeidiM;Guo,Jinbai;Ellis,Sarah;Polymenis,Michael
细胞分裂的启动是由一个不可逆的开关控制的。在酿酒酵母中，Sic 1 p蛋白（有丝分裂细胞周期蛋白/细胞周期蛋白依赖性激酶复合物的抑制剂）的降解发生在DNA复制开始之前，在称为START的点。Sic 1 p被多种激酶磷酸化，这些激酶可以不同地影响Sic 1 p的稳定性。稳定Sic 1 p的磷酸化是如何逆转的尚不清楚。在这里，我们表明，Dcr 2 p磷酸酶功能和物理相互作用与Sic 1 p。Dcr 2 p的过度表达使Sic 1 p不稳定，并导致与不稳定的Sic 1 p相关的表型，如基因组不稳定性。我们的研究结果确定了一种影响Sic 1 p稳定性的新因子，可能有助于触发细胞分裂启动的机制。
Initiation of cell division is controlled by an irreversible switch. In Saccharomyces cerevisiae degradation of the Sic1p protein, an inhibitor of mitotic cyclin/cyclin-dependent kinase complexes, takes place before initiation of DNA replication, at a point called START. Sic1p is phosphorylated by multiple kinases, which can differentially affect the stability of Sic1p. How phosphorylations that stabilize Sic1p are reversed is unknown. Here we show that the Dcr2p phosphatase functionally and physically interacts with Sic1p. Over-expression of Dcr2p destabilizes Sic1p and leads to phenotypes associated with destabilized Sic1p, such as genome instability. Our results identify a novel factor that affects the stability of Sic1p, possibly contributing to mechanisms that trigger initiation of cell division.