cpc-1, the general regulatory gene for genes of amino acid biosynthesis in Neurospora crassa, is differentially expressed during the asexual life cycle
cpc-1, the general regulatory gene for genes of amino acid biosynthesis in Neurospora crassa, is differentially expressed during the asexual life cycle
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DOI：
10.1128/mcb.11.2.928-934.1991
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发表时间：
1991-02
影响因子：
5.3
通讯作者：
D. Ebbole;Janet L. Paluh;Michael Plamann;Matthew S. Sachs;Charles Yanofsky
中科院分区：
文献类型：
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作者：
D. Ebbole;Janet L. Paluh;Michael Plamann;Matthew S. Sachs;Charles Yanofsky
Cpci是粗粗脉孢霉氨基酸生物合成基因跨途径调控所需的主要调控蛋白，其结构域与酿酒酵母中相应的通用调控因子Gcn4的DNA结合域相似。我们检测了体外合成的CPC1和粗毛藻全细胞提取物中的CPC1的结合作用。两种来源的CpCI都能与DNA序列5‘-ATGACTCAT-3’结合，这也是Gcn4的首选识别序列，免疫印迹证实CPC1是提取物中DNA结合活性的来源。体外合成的含有CPC1的DNA复合体与菌丝体提取液中的CPC1的迁移率略有差异。对粗毛拟青霉不同无性发育阶段提取液的分析表明，CPC1在孢子萌发后即刻和菌丝生长早期含量丰富，但随后稀少。通过施加氨基酸饥饿，CPC1水平可以在任何时候增加。CPC1反应元件的拷贝位于几个受交叉途径调控的基因的上游，包括CPC-1本身。
CPCI, the principal regulatory protein required for cross-pathway control of amino acid biosynthetic genes in Neurospora crassa, contains a domain similar to the DNA-binding domain of GCN4, the corresponding general regulator in Saccharomyces cerevisiae. We examined binding by CPC1 synthesized in vitro and by CPC1 present in N. crassa whole-cell extracts. CPCI from both sources was shown to bind to the DNA sequence 5'-ATGACTCAT-3', which is also the preferred recognition sequence of GCN4, CPC1 was confirmed as the source of DNA-binding activity in extracts by immunoblotting. Slightly mobility differences between DNA complexes containing CPCI synthesized in vitro and CPC1 in mycelial extracts were observed. Analyses of N. crassa extracts from different stages of asexual development revealed that CPC1 was abundant immediately following spore germination and through early mycelial growth but was scarce subsequently. CPC1 levels could be increased at any time by imposing amino acid starvation. Copies of the CPC1 response element are located upstream of several genes regulated by cross-pathway control, including cpc-1 itself.