Standardization of Laboratory Methods for the PERCH Study.
Standardization of Laboratory Methods for the PERCH Study.
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DOI：
10.1093/cid/cix081
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发表时间：
2017-06-15
期刊：
影响因子：
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通讯作者：
Murdoch DR
中科院分区：
文献类型：
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作者：
Driscoll AJ;Karron RA;Morpeth SC;Bhat N;Levine OS;Baggett HC;Brooks WA;Feikin DR;Hammitt LL;Howie SRC;Knoll MD;Kotloff KL;Madhi SA;Scott JAG;Thea DM;Adrian PV;Ahmed D;Alam M;Anderson TP;Antonio M;Baillie VL;Dione M;Endtz HP;Gitahi C;Karani A;Kwenda G;Maiga AA;McClellan J;Mitchell JL;Morailane P;Mugo D;Mwaba J;Mwansa J;Mwarumba S;Nyongesa S;Panchalingam S;Rahman M;Sawatwong P;Tamboura B;Toure A;Whistler T;O'Brien KL;Murdoch DR
儿童健康肺炎病原学研究在7个不同的研究中心进行，并依赖于标准化的临床和实验室方法对肺炎病原学数据进行准确和有意义的解释。血液、呼吸道标本和尿液采集自1-59个月因重度或极重度肺炎住院的儿童和同龄无重度肺炎的社区对照组，并进行了广泛的实验室诊断检测。所有研究中心均采用标准化检测算法和标准操作规程。研究中心实验室接受了核心检测方法的统一培训、设备和试剂。通过例行电话会议、面对面会议、现场监测访问以及内部和外部质量保证测试，进一步确保了标准化。在中心参考实验室进行了针对性确证性检测和专门检测试剂盒检测。
The Pneumonia Etiology Research for Child Health study was conducted across 7 diverse research sites and relied on standardized clinical and laboratory methods for the accurate and meaningful interpretation of pneumonia etiology data. Blood, respiratory specimens, and urine were collected from children aged 1–59 months hospitalized with severe or very severe pneumonia and community controls of the same age without severe pneumonia and were tested with an extensive array of laboratory diagnostic tests. A standardized testing algorithm and standard operating procedures were applied across all study sites. Site laboratories received uniform training, equipment, and reagents for core testing methods. Standardization was further assured by routine teleconferences, in-person meetings, site monitoring visits, and internal and external quality assurance testing. Targeted confirmatory testing and testing by specialized assays were done at a central reference laboratory.