Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces
Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone (PEEK) surfaces
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DOI：
10.1007/s10856-005-2539-z
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发表时间：
2005-07-01
影响因子：
3.7
通讯作者：
Rueger, JM
中科院分区：
文献类型：
--
作者：
Briem, D;Strametz, S;Rueger, JM
聚醚醚酮（PEEK）是一种合成聚合物，具有合适的生物力学和稳定的化学性质，这使得其在用作内假体材料和韧带置换方面具有吸引力。然而，化学表面惰性并不占良好的界面生物相容性，PEEK需要表面改性之前，其在vivo.In本实验研究的过程中，我们分析了PEEK表面的等离子体处理对原代成纤维细胞和成骨细胞的细胞增殖和分化的影响。此外，我们研究了诱导微结构细胞生长的可能性与等离子体诱导的化学micropatterning的表面上。我们能够证明，PEEK的表面处理与低温等离子体对成纤维细胞的增殖有显着的影响。根据表面处理，增殖速率可以被刺激或抑制。通过检测碱性磷酸酶、胶原1和矿化细胞外基质作为成骨细胞分化的参数，检测的材料显示出与市售聚合物细胞培养材料如组织培养聚苯乙烯（TCPS）相当的结果。进一步的微结构化的细胞生长成功地产生在微图案化的PEEK箔，这可能是一个未来的工具，生物人工系统应用组织engineering的方法，这些结果表明，化学惰性材料，如PEEK可以通过等离子体技术的方法进行专门修改，以提高生物相容性。(C)2005 Springer Science + Business Media，Inc.
Polyetheretherketone (PEEK) is a synthetic polymer with suitable biomechanical and stable chemical properties, which make it attractive for use as an endoprothetic material and for ligamentous replacement. However, chemical surface inertness does not account for a good interfacial biocompatibility, and PEEK requires a surface modification prior to its application in vivo.In the course of this experimental study we analyzed the influence of plasma treatment of PEEK surfaces on the cell proliferation and differentiation of primary fibroblasts and osteoblasts. Further we examined the possibility of inducing microstructured cell growth on a surface with plasma-induced chemical micropatterning.We were able to demonstrate that the surface treatment of PEEK with a low-temperature plasma has significant effects on the proliferation of fibroblasts. Depending on the surface treatment, the proliferation rate can either be stimulated or suppressed. The behavior of the osteoblasts was examined by evaluating differentiation parameters.By detection of alkaline phosphatase, collagen 1, and mineralized extracellular matrix as parameters for osteoblastic differentiation, the examined materials showed results comparable to commercially available polymer cell culture materials such as tissue culture polystyrene (TCPS). Further microstructured cell growth was produced successfully on micropatterned PEEK foils, which could be a future tool for bioartificial systems applying the methods of tissue engineering.These results show that chemically inert materials such as PEEK may be modified specifically through the methods of plasma technology in order to improve biocompatibility. (C) 2005 Springer Science + Business Media, Inc.