Biochemically Triggered Heat and Drought Stress Tolerance in Rice by Proline Application
Biochemically Triggered Heat and Drought Stress Tolerance in Rice by Proline Application
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DOI：
10.1007/s00344-020-10095-3
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发表时间：
2020-03-27
影响因子：
4.8
通讯作者：
Khan, Haroon Zaman
中科院分区：
文献类型：
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作者：
Hanif, Sajid;Saleem, Muhammad Farrukh;Khan, Haroon Zaman
非生物胁迫是气候变化条件下作物可持续生产的主要胁迫因素。水稻生殖和营养阶段的高温和干旱胁迫导致其产量大幅度下降。脯氨酸作为一种多功能氨基酸，被用于减轻植物的多种生物和非生物胁迫。2018年夏季，在巴基斯坦费萨拉巴德农业大学农学院温室/筛房进行了盆栽试验，以检验叶面施用脯氨酸对缓解高温和干旱胁迫对水稻的同时影响的有效性。试验采用完全随机设计，分为3个重复。在花期以干旱胁迫、热胁迫和热加干旱胁迫为主要胁迫因子，外加不同浓度脯氨酸（不喷脯氨酸/水、10、20和30 mM）作为次要胁迫因子。对照组（没有施加压力）维持比较。胁迫处理：花期干旱、高温和热加干旱胁迫抑制了抗氧化剂、渗透保护剂和叶绿素含量的产生，导致丙二醛含量过量。外源脯氨酸增加了植株超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、总可溶性蛋白（TSP）活性、叶片脯氨酸和甜菜碱含量，降低了脂质过氧化作用，提高了叶绿素含量，最终提高了单株产量。与单独施用高温或干旱胁迫相比，同时施用高温和干旱胁迫更危险，30毫米脯氨酸的施用对胁迫的缓解作用最大。
Abiotic stresses are the prime coercion to sustainable crop production in changing climate scenario. Heat and drought stresses at reproductive as well as vegetative stages of rice cause extensive reduction in its yield. Being a multifunctional amino acid, proline is being used to diminish numerous biotic and abiotic stresses of plants. A pot experiment was conducted in summer season 2018, to check the effectiveness of foliar applied proline in mitigating the concurrent effects of heat and drought stresses on rice, at greenhouse/screenhouse of Faculty of Agriculture, University of Agriculture, Faisalabad, Pakistan. Experiment was carried out under completely randomized design with split arrangement having three replications. Stress was imposed at anthesis with treatments viz. drought stress, heat stress and heat plus drought stress as a main factor, and various levels of exogenously applied proline viz. no proline/water spray, 10, 20 and 30 mM concentrations were maintained as subfactor. A control (no stress imposed) was upheld for comparison. Stress treatments: drought, heat and heat plus drought stress at anthesis stage depressed the production of antioxidants, osmoprotectants and chlorophyll contents while causing overproduction of malondialdehyde content. Exogenous proline application upregulated activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total soluble proteins (TSP), leaf proline and glycine betaine contents and diminution in lipid peroxidation resulting into improvement in chlorophyll contents and eventually yield per plant. Concurrent heat and drought stresses were more perilous as compared to individually applied heat or drought stress and 30 mM proline application gave maximum alleviation against stress.