TaNF-YB3 is involved in the regulation of photosynthesis genes in Triticum aestivum
TaNF-YB3 is involved in the regulation of photosynthesis genes in Triticum aestivum
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DOI：
10.1007/s10142-011-0212-9
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发表时间：
2011-02
影响因子：
2.9
通讯作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
中科院分区：
文献类型：
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作者：
Troy J. Stephenson;Troy J. Stephenson;C. McIntyre;C. Collet;G. Xue
核因子 Y (NF-Y) 转录因子是由三个亚基组成的异源三聚体：NF-YA、NF-YB 和 NF-YC。植物中的三个亚基均由具有差异表达谱的多个基因编码，这意味着植物中 NF-Y 亚基成员的功能特化。在这项研究中，我们研究了 NF-YB 成员在光介导的光合作用基因调控中的作用。我们使用定量 RT-PCR 鉴定了小麦 (TaNF-YB3&7) 中的两个 NF-YB 成员，它们在叶子和幼苗中受光显着上调。对多个 Affymetrix 小麦基因组阵列数据集的全基因组共表达分析表明，TaNF-YB3 共表达转录本高度富集基因本体术语光合作用。组成型过表达TaNF-YB3的转基因小麦品系的叶片叶绿素含量、光合作用速率和早期生长速率显着增加。定量RT-PCR分析表明，转基因小麦品系中许多TaNF-YB3共表达转录物的表达水平升高。转基因小麦叶片中编码谷氨酰-tRNA还原酶的TaGluT mRNA水平显着增加，谷氨酰-tRNA还原酶催化叶绿素生物合成途径的限速步骤。还观察到编码叶绿素 a/b 结合蛋白（Lhca4 和 Lhcb4）和光系统 I 反应中心亚基（K 亚基和 N 亚基）的四个光合机构基因以及编码叶绿体 ATP 合酶 γ 亚基的基因在转基因植物叶子中的表达水平显着增加。这些结果表明TaNF-YB3参与了小麦中多个光合作用基因的正向调节。
Nuclear factor Y (NF-Y) transcription factor is a heterotrimer comprised of three subunits: NF-YA, NF-YB and NF-YC. Each of the three subunits in plants is encoded by multiple genes with differential expression profiles, implying the functional specialisation of NF-Y subunit members in plants. In this study, we investigated the roles of NF-YB members in the light-mediated regulation of photosynthesis genes. We identified two NF-YB members fromTriticum aestivum(TaNF-YB3&7) which were markedly upregulated by light in the leaves and seedling shoots using quantitative RT-PCR. A genome-wide coexpression analysis of multiple Affymetrix Wheat Genome Array datasets revealed thatTaNF-YB3-coexpressed transcripts were highly enriched with the Gene Ontology termphotosynthesis. Transgenic wheat lines constitutively overexpressingTaNF-YB3had a significant increase in the leaf chlorophyll content, photosynthesis rate and early growth rate. Quantitative RT-PCR analysis showed that the expression levels of a number ofTaNF-YB3-coexpressed transcripts were elevated in the transgenic wheat lines. The mRNA level ofTaGluTRencoding glutamyl-tRNA reductase, which catalyses the rate-limiting step of the chlorophyll biosynthesis pathway, was significantly increased in the leaves of the transgenic wheat. Significant increases in the expression level in the transgenic plant leaves were also observed for four photosynthetic apparatus genes encoding chlorophyll a/b-binding proteins (Lhca4 and Lhcb4) and photosystem I reaction centre subunits (subunit K and subunit N), as well as for a gene coding for chloroplast ATP synthase γ subunit. These results indicate thatTaNF-YB3is involved in the positive regulation of a number of photosynthesis genes in wheat.