Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice
Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice
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验证 6 号染色体短臂上负责稻壳硅含量和产量性状遗传变异的片段
DOI：
10.1007/s10681-007-9501-8
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发表时间：
2008-04-01
期刊：
影响因子：
1.9
通讯作者：
Zhuang, Jie-Yun
中科院分区：
文献类型：
--
作者：
Dai, Wei-Min;Zhang, Ke-Qin;Zhuang, Jie-Yun
水稻是一种典型的硅积累植物，硅对水稻的有益作用早已被人们所认识。利用2003年育成的244个籼稻杂交组合珍汕97 B/密阳46的重组自交系（RIL），检测到4个影响船体硅含量的QTL。其中qHUS-6的效应最大，同一区间对同一群体的产量性状也有显著的效应。本研究的主要目的是验证该地区QTL对HUS和产量性状的影响。同一个RIL群体和另一个异质性较低的RIL群体在2004年生长。QTL qHUS-6对船体硅含量有显著的加性效应，且方向一致。从同一杂交组合的重组自交系中选出一个剩余的杂合系，得到15个F-2：3系，它们的差异仅在于第6染色体短臂上从RM 587延伸到RM 6119的2.15-Mb片段。在这些品系中，qHUS-6表现出主要效应，先前在同一地区检测到的产量性状的QTL也表现出主要效应。2003年检测到的另外两个HUS QTL qHUS-1-1和qHUS-1-2在2004年珍山97 B/密阳46 RIL群体中也具有一致的效应。本研究验证了3个候选区域，可用于精细定位水稻硅素敏感性QTL，确定硅素含量与产量性状的遗传关系。
Rice is a typical silicon-accumulating plant and the beneficial effect of silicon on rice has long been recognized. In a previous study using 244 recombinant inbred lines (RILs) of an indica rice cross, Zhenshan 97B/Milyang 46 grown in 2003, four QTLs were detected for hull silicon content. QTL qHUS-6 had the largest effect among these, and the same interval also had significant effects on yield traits in the same population. The primary objective of this study was to validate the QTL effect in this region on HUS and yield traits. The same RIL population and another RIL population of lower heterogeneity were grown in 2004. QTL qHUS-6 was found to have significant additive effects on hull silicon content with a consistent direction in the two populations. From a residual heterozygous line selected from RILs of the same cross, 15 F-2:3 lines that differed only in a 2.15-Mb segment extending from RM587 to RM6119 on the short arm of chromosome 6 were derived. In these lines, qHUS-6 displayed a major effect, so did QTLs for yield traits previously detected in the same region. Two more QTLs for HUS detected in 2003, qHUS-1-1 and qHUS-1-2, also had consistent effects in the Zhenshan 97B/Milyang 46 RIL population in 2004. Thus this study verified three candidate regions for fine mapping HUS QTLs and determining the genetic relationship between silicon content and yield traits in rice.