Rice stripe virus hitchhikes the vector insect vitellogenin ligand-receptor pathway for ovary entry
Rice stripe virus hitchhikes the vector insect vitellogenin ligand-receptor pathway for ovary entry
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水稻条纹病毒搭便车媒介昆虫卵黄原配体-受体途径进入卵巢
DOI：
10.1098/rstb.2018.0312
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发表时间：
2019-03-04
影响因子：
6.3
通讯作者：
Fang, Rongxiang
中科院分区：
文献类型：
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作者：
Huo, Yan;Yu, Yuanling;Fang, Rongxiang
已知植物虫媒病毒通过内吞作用感染昆虫载体细胞；然而，介导内吞作用的细胞受体尚未明确定义。在我们最近发表的工作和本研究中，通过阐明灰飞虱中水稻条纹病毒（RSV）的垂直传播机制，我们为虫媒病毒如何进入昆虫生殖系细胞提供了一个新的范例。该病毒不是与病毒受体直接相互作用，而是与分泌的配体蛋白结合，搭上配体-受体途径的便车以实现进入细胞。卵黄蛋白原 (Vg) 是胚胎发育不可或缺的蛋白质，在卵巢外合成，并通过 Vg 受体 (VgR) 介导的内吞作用被生殖细胞吸收。在揭示RSV通过与血淋巴中昆虫Vg的特异性分子相互作用入侵灰飞虱卵巢后，本研究探讨了VgR在介导RSV入侵生殖细胞中的功能，进一步证实了配体受体介导的病毒细胞入侵机制。了解病毒进入载体的卵巢途径将有助于找到合适的措施来阻止病毒的跨代传播。本文是主题“生物信号揭示智能害虫管理”的一部分。
It is known that plant arboviruses infect insect vector cells by endocytosis; however, the cellular receptors that mediate endocytosis have not been well defined. In our recently published work and this study, by clarifying the vertical transmission mechanism of Rice stripe virus (RSV) in Laodelphax striatellus, we provide a novel paradigm for how arboviruses enter insect germ-line cells. Instead of direct interaction with a viral receptor, the virus binds to a secreted ligand protein, hitchhiking the ligand-receptor pathway to achieve cell entry. Vitellogenin (Vg) is an indispensable protein for embryo development that is synthesized extra-ovarially and taken up by germ-line cells through Vg receptor (VgR)-mediated endocytosis. After revealing that RSV invades L. striatellus ovary by a specific molecular interaction with the insect Vg in haemolymph, this study addressed VgR's function in mediating the RSV invasion of the germarium nurse cells, further confirming the ligand's receptor-mediated viral cell-invasion mechanism. Understanding the viral ovary-entry pathways in vectors will help to find suitable measures to block the trans-generation transmission of the viruses. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.