Cytokines in the blood and semen of infertile patients.
Cytokines in the blood and semen of infertile patients.
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DOI：
10.5114/ceji.2015.54596
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Kurpisz M
中科院分区：
文献类型：
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作者：
Havrylyuk A;Chopyak V;Boyko Y;Kril I;Kurpisz M
细胞因子是重要的免疫介质，可参与男性生殖道的许多过程，包括作为男性性腺的免疫调节元件。本研究的目的是：1)检测不育男性对照组和亚组的促炎性和抗炎性细胞因子水平；2)为男性不育症的诊断提供细胞因子水平测定的实用建议。对82名男性进行了观察：健康对照组（n = 27）和不育患者（n = 55）。男性不育症组进一步细分为精索静脉曲张（n = 22）、特发性不育症（n = 13）和复发性自然流产夫妇的伴侣（RSA; n = 20）。精液分析按照世卫组织标准进行。细胞因子白介素1β (IL-1β)、IL-6、IL-10、IL-18；采用定量ELISA法测定血清和精浆中肿瘤坏死因子α (TNF-α)、干扰素g （IFN-g）和转化生长因子β1 （TGF-β1）的含量。男性不育症的一个有趣的标志似乎是TGF-β1（血）在特发性不育症男性和RSA组显著升高。此外，特发性不育症组TGF-β1升高，IL-10、IL-18、IFN-g（血）显著升高，IL-1β显著降低，精浆中IFN-g显著升高。我们可以假设不同背景的不孕症患者新的细胞因子微模式。因此，应扩大精浆中循环细胞因子：IL-1β、IL-10、IL-18、TGF-β1、IFN-g和IL-1β、IFN-g和TGF-β1对男性不育症具体类型的评价。
Cytokines have been important mediators of the immunity and can be involved in numerous processes in the male genital tract including acting as immunomodulatory elements within the male gonad. The aims of this study were: 1) to detect pro- and anti-inflammatory cytokine levels in the control group and subgroups of infertile men; and 2) to set up the practical recommendations concerning determination of cytokine levels for the male infertility diagnosis. Observations were performed in a group of 82 men: healthy controls (n = 27) and infertile patients (n = 55). The male infertility group was further subdivided into patients with: varicocele (n = 22), idiopathic infertility (n = 13) and partners of couples with recurrent spontaneous abortion (RSA; n = 20). Semen analysis was determined following WHO criteria. The cytokine interleukin 1β (IL-1β), IL-6, IL-10, IL-18; tumor necrosis factor α (TNF-α), interferon g (IFN-g) and transforming growth factor β1 (TGF-β1) contents in serum and seminal plasma were determined by quantitative ELISA. An interesting marker of male infertility appears to be TGF-β1 (blood) significantly elevated in idiopathically infertile males and in the RSA group. Besides elevated TGF-β1 in a group of idiopathic infertility significantly elevated IL-10, IL-18, IFN-g (blood) and statistically decreased IL-1β while increased IFN-g were revealed in seminal plasma compared to healthy controls. We may postulate novel cytokine micropatterns for patients with different background of infertility. Therefore, circulating cytokines: IL-1β, IL-10, IL-18, TGF-β1, IFN-g and IL-1β, IFN-g and TGF-β1 in seminal plasma should be extended in evaluation of specific types of male infertility.