A preliminary approach to the repair of myocardial infarction using adipose tissue-derived stem cells encapsulated in magnetic resonance-labelled alginate microspheres in a porcine model
A preliminary approach to the repair of myocardial infarction using adipose tissue-derived stem cells encapsulated in magnetic resonance-labelled alginate microspheres in a porcine model
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DOI：
10.1016/j.ejpb.2012.11.028
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发表时间：
2013-05-01
影响因子：
4.9
通讯作者：
Fernanda Martin-Cancho, Maria
中科院分区：
文献类型：
--
作者：
Guadalupe Gomez-Mauricio, Remedios;Acarregui, Argia;Fernanda Martin-Cancho, Maria
脂肪组织源性干细胞（ASCs）具有自我更新、多能性和高增殖能力的特性，可用于缺血性损伤后心室功能的治疗。然而，由于细胞在靶部位的保留率低，它们的治疗用途受到限制。为了解决这个问题，我们开发了带有Endorem (R)（磁胶囊）标记的半透膜微胶囊，在维持细胞内部微环境的同时为细胞提供机械和免疫免疫保护。此外，这些颗粒可以通过磁共振成像（MRI）跟踪注射细胞在体内的存在和迁移。结果表明，培养21天后，包被磁胶囊的细胞存活率与包被普通微胶囊的细胞存活率相近。MRI证实，在未包裹的Endorem (R)标记细胞中，氧化铁标记的强度逐渐丧失，而在整个研究期间，都检测到磁胶囊，这表明当细胞被包裹在磁胶囊内时，心肌中的细胞潴留得到改善。为了进一步评估治疗对整体心功能的影响，MRI测定梗死面积和左心室射血分数（LVEF）。体内实验结果显示，两组间心率和心输出量差异无统计学意义。总之，在整个研究期间，封闭在磁胶囊内的细胞表现出适当的活力，并在体内被检测到。进一步的研究将评估是否增加细胞负载颗粒可能有助于改善治疗效果。(C) 2012 Elsevier B.V.版权所有
Adipose tissue-derived stem cells (ASCs) have properties of self-renewal, pluripotency and high proliferative capability that make them useful for the treatment of cardiac ventricular function following ischaemic injury. However, their therapeutic use is limited due to the low retention of the cells at the targeted site. To address this issue, we developed semipermeable membrane microcapsules labelled with Endorem (R) (magnetocapsules) that provide mechanical and immunological immune protection to the cells while maintaining internal cell microenvironment. In addition, the particles allow tracking the presence and migration of injected cells in vivo by Magnetic Resonance Imaging (MRI). Results indicate that after 21 days in culture, the cells encapsulated in the magnetocapsules showed similar viabilities than cells encapsulated in conventional microcapsules. MRI confirmed a gradual loss of the intensity of the iron oxide label in the non-encapsulated Endorem (R) labelled cells, while magnetocapsules were detected throughout the study period, suggesting that cell retention in the myocardium is improved when cells are enclosed within the magnetocapsules. To further evaluate treatment's effect on global cardiac function, MRI determination of infarct size and left ventricular ejection fraction (LVEF) was performed. In vivo results showed no statistically significant differences in heart rate and cardiac output between treatment groups. In conclusion, cells enclosed within magnetocapsules have shown suitable viability and have been detected in vivo throughout the study period. Further studies will evaluate whether increasing cell loading with the particles may help to improve the therapeutic results. (C) 2012 Elsevier B.V. All rights reserved.