Comparison of the influenza virus-specific effector and memory C-cell responses to immunization of children and adults with live attenuated or inactivated influenza virus vaccines
Comparison of the influenza virus-specific effector and memory C-cell responses to immunization of children and adults with live attenuated or inactivated influenza virus vaccines
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DOI：
10.1128/jvi.01957-06
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发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Greenberg, Harry B.
中科院分区：
文献类型：
--
作者：
Sasaki, Sanae;Jaimes, Maria C.;Greenberg, Harry B.
针对流感病毒感染和流感病毒疫苗接种的细胞免疫应答尚未得到严格的特征描述。我们对儿童和成人接种减毒活流感疫苗（LAIV）或灭活流感疫苗（TIV）后的效应B细胞和记忆B细胞应答进行了量化，并将其与抗体应答进行了比较。在幼儿（6个月至4岁）、大龄儿童（5至9岁）和成人免疫接种后的第0天、第7 - 12天以及第27 - 42天采集外周血单个核细胞。使用酶联免疫斑点试验检测流感病毒特异性效应免疫球蛋白A（IgA）和IgG循环抗体分泌细胞（ASC）以及受刺激的记忆B细胞。在接种TIV和LAIV后的7 - 12天检测到循环流感病毒特异性IgG和IgA ASC。79%或更多的成人和大龄儿童有可检测到的IgG ASC应答，而29% - 53%的受试者检测到IgA ASC应答。成人对LAIV免疫接种的IgG ASC应答率显著高于标准血清抗体检测方法所测得的应答率（中和试验和血凝抑制试验分别为26.3%和15.8%）。与大龄儿童和成人相比，幼儿的IgG ASC和血清抗体应答相对较低。在儿童和成人中，TIV（而非LAIV）显著增加了免疫接种后27 - 42天检测到的循环流感病毒特异性记忆B细胞的百分比。总之，尽管两种流感疫苗都有效，但我们发现成人和大龄儿童接种LAIV或TIV后引发的B细胞和抗体应答存在显著差异，幼儿和大龄组之间也存在差异。
Cellular immune responses to influenza virus infection and influenza virus vaccination have not been rigorously characterized. We quantified the effector and memory B-cell responses in children and adults after administration of either live attenuated (LAW) or inactivated (TIV) influenza virus vaccines and compared these to antibody responses. Peripheral blood mononuclear cells were collected at days 0, 7 to 12, and 27 to 42 after immunization of younger children (6 months to 4 years old), older children (5 to 9 years old), and adults. Influenza virus-specific effector immunoglobulin A (IgA) and IgG circulating antibody-secreting cells (ASC) and stimulated memory B cells were detected using an enzyme-linked immunospot assay. Circulating influenza virus-specific IgG and IgA ASC were detected 7 to 12 days after TIV and after LAIV immunization. Seventy-nine percent or more of adults and older children had demonstrable IgG ASC responses, while IgA ASC responses were detected in 29 to 53% of the subjects. The IgG ASC response rate to LAIV immunization in adults was significantly higher than the response rate measured by standard serum antibody assays (26.3% and 15.8% by neutralization and hemagglutination inhibition assays, respectively). IgG ASC and serum antibody responses were relatively low in the younger children compared to older children and adults. TIV, but not LAIV, significantly increased the percentage of circulating influenza virus-specific memory B cells detected at 27 to 42 days after immunization in children and adults. In conclusion, although both influenza vaccines are effective, we found significant differences in the B-cell and antibody responses elicited after LAW or TIV immunization in adults and older children and between young children and older age groups.