Detection of HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor
Detection of HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor
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DOI：
10.1016/j.snb.2010.01.067
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发表时间：
2010-04-08
影响因子：
8.4
通讯作者：
Fan, Xudong
中科院分区：
文献类型：
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作者：
Gohring, John T.;Dale, Paul S.;Fan, Xudong
蛋白质生物标志物最近在检测、定量和监测侵袭性类型乳腺癌中的作用方面进行了大量研究。在这项工作中，我们描述了一种新的，无标记的方法，用于检测人类血清样品中的HER 2胞外结构域乳腺癌生物标志物，使用光流体环谐振器（OFRR）。OFRR结合了微流体和光学环形谐振器传感技术，可在小型低成本平台上实现快速无标记检测。在本研究中，将HER 2蛋白以不同浓度加标至血清中。结果表明，OFRR能够检测HER 2在30分钟内在血清中的医学相关浓度范围从13至100 ng/mL。我们的工作将导致一种设备，可用作一种工具，用于监测疾病的进展，在低成本的传感设置。(c)2010爱思唯尔有限公司版权所有。
Protein biomarkers have recently been heavily researched in their roles in the detection, quantification, and monitoring of aggressive types of breast cancer. In this work, we describe a novel, label-free approach for detecting the HER2 extra-cellular domain breast cancer biomarker in human serum samples using the opto-fluidic ring resonator (OFRR). The OFRR incorporates microfluidics and optical ring resonator sensing technology to achieve rapid label-free detection in a small and low-cost platform. In this study, HER2 proteins were spiked in serum at varying concentrations. Results show that the OFRR is able to detect HER2 at medically relevant concentrations in serum ranging from 13 to 100 ng/mL in 30 min. Our work will lead to a device that can be used as a tool for monitoring disease progression in a low-cost sensing setup. (c) 2010 Elsevier B.V. All rights reserved.