Mitochondrial content reflects oocyte variability and fertilization outcome
Mitochondrial content reflects oocyte variability and fertilization outcome
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DOI：
10.1016/j.fertnstert.2005.09.017
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发表时间：
2006-03-01
影响因子：
6.7
通讯作者：
St John, JC
中科院分区：
文献类型：
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作者：
Santos, TA;El Shourbagy, S;St John, JC
目的：确定受精成功和失败患者卵母细胞中线粒体DNA（mtDNA）的含量。设计：通过实时聚合酶链反应（PCR）分析受精和未受精卵母细胞和胚胎中mtDNA的含量。设置：大学医院不孕症和研究中心。患者：54名寻求不孕症治疗的妇女。干预：无。主要结果测量：共142个受精和未受精的卵母细胞分为三个主要组。I组包括21例患者的35个受精卵; 11组，36例患者的65个未受精卵; 111组，23例患者的42个退化卵。结果：受精卵和未受精卵的mtDNA拷贝数分别为250，454和163，698。男性因素与女性因素不育未受精卵之间、原因不明不育组与女性因素不育组之间mtDNA拷贝数差异有统计学意义。退化卵母细胞组的平均拷贝数为44，629，与该组中的其他亚组显著不同。结论：线粒体DNA含量对受精结果至关重要，是卵母细胞质量的重要标志，可以解释一些受精失败的病例。
Objective: To determine the content of mitochondrial DNA (mtDNA) in oocytes from a range of patients with fertilization success and failure.Design: Analysis of mtDNA content in fertilized and unfertilized oocytes and embryos by real-time polymerase chain reaction (PCR).Setting: University hospital infertility and research center.Patient(s): Fifty-four women seeking treatment for infertility.Intervention(S): None.Main Outcome Measure(s): A total of 142 fertilized and unfertilized oocytes were classified into three main groups. Group I consisted of 35 fertilized oocytes from 21 patients; group 11, 65 unfertilized oocytes from 36 patients; and group 111, 42 degenerate oocytes from 23 patients. Mitochondrial DNA content was determined by SYBR Green real-time PCR-based assay.Result(s): The mean mtDNA copy number for the fertilized oocytes was 250,454, whereas for the unfertilized group it was 163,698. There were significant differences for mtDNA copy number between the male factor and female factor infertility unfertilized oocytes and between the unexplained infertility and female factor infertility groups. The mean copy number for the degenerate oocyte group was 44,629, which was significantly different from the other subdivisions in this group.Conclusion(s): Mitochondrial DNA content is critical to fertilization outcome and serves as an important marker of oocyte quality, explaining some cases of fertilization failure.