AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: Characterization, transposon-induced allelic variation and meiosis-associated expression
AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: Characterization, transposon-induced allelic variation and meiosis-associated expression
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DOI：
10.1046/j.1365-313x.1997.11010001.x
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发表时间：
1997-01-01
期刊：
影响因子：
7.2
通讯作者：
Jones, JDG
中科院分区：
文献类型：
--
作者：
Klimyuk, VI;Jones, JDG
根据酵母DMC 1与百合LIM 15减数分裂特异基因的同源性，设计简并PCR引物，扩增拟南芥DMC 1基因（AtDMC 1）。对AtDMC 1基因组DNA（8 kb）进行测序，并通过逆转录酶-聚合酶链反应（RT-PCR）和5'和3' RACE（cDNA末端快速扩增）对转录本进行表征。AtDMC 1基因包含15个外显子和14个内含子。RNA原位杂交分析表明AtDMC 1的表达仅限于花药中的花粉母细胞和胚珠中的大孢子母细胞。AtDMC 1启动子融合到GUS报告基因，并赋予减数分裂相关的表达在男性和女性的花谱系。比较从兰茨贝格直立生态型分离的AtDMC 1与其哥伦比亚等位基因ArLIM 15，揭示了在ArLIM 15的启动子区域内存在1874 bp的转座子样元件。RT-PCR分析表明AtDMC 1和ArLIM 15的表达水平相似。AtDMC 1启动子的可能用途进行了讨论。
Based on homologies between the yeast DMC1 and the lily LIM15 meiosis-specific genes, degenerate PCR primers were designed that amplified the Arabidopsis DMC1 gene (AtDMC1). AtDMC1 genomic DNA (8 kb) was sequenced, and the transcript was characterized by reverse transcriptase-polymerase chain reaction (RT-PCR) and by 5' and 3' RACE (rapid amplification of cDNA ends). The AtDMC1 gene contains 15 exons and 14 introns. RNA in situ hybridization analysis showed that expression of the AtDMC1 is restricted to pollen mother cells in anthers and to megaspore mother cells in ovules. The AtDMC1 promoter was fused to the GUS reporter gene, and conferred meiosis associated expression in both male and female floral lineages. Comparison of AtDMC1 isolated from Landsberg erecta ecotype to its Columbia allele ArLIM15, revealed the presence of a 1874 bp transposon-like element within the promoter region of ArLIM15. RT-PCR analysis showed that the expression levels of AtDMC1 and ArLIM15 are similar. Possible uses for the AtDMC1 promoter are discussed.