Nanotopography-guided tissue engineering and regenerative medicine.
Nanotopography-guided tissue engineering and regenerative medicine.
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DOI：
10.1016/j.addr.2012.07.014
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发表时间：
2013-04
影响因子：
16.1
通讯作者：
Suh KY
中科院分区：
文献类型：
--
作者：
Kim HN;Jiao A;Hwang NS;Kim MS;Kang DH;Kim DH;Suh KY
人体组织是多种细胞类型的复杂集合体，嵌入细胞外基质（ECM）的复杂和明确的结构中。ECM的组织通常是从纳米到宏观的层次结构，许多蛋白质形成大规模结构，特征尺寸高达数百微米。受ECM的这些自然设计的启发，在过去的几十年中，纳米形貌引导的方法已经越来越多地被研究。结果表明，纳米形貌本身可以在体外激活组织特异性功能，以及在移植后促进体内组织再生。在这篇综述中，我们提供了一个广泛的分析，最近的努力，模仿功能性纳米结构在体外改善组织工程和再生的受伤和受损组织。我们首先描述了各种纳米结构在人体组织中的作用，每个组织特异性功能。然后，我们描述了各种制作方法的图案化原理和材料特性。最后，我们总结了纳米形貌在各种组织中的应用，根据其功能分为四种类型：保护性组织、机械敏感组织、电活性组织和剪切应力敏感组织。最后简要讨论了一些局限性和未来的挑战。
Human tissues are intricate ensembles of multiple cell types embedded in complex and well-defined structures of the extracellular matrix (ECM). The organization of ECM is frequently hierarchical from nano to macro, with many proteins forming large scale structures with feature sizes up to several hundred microns. Inspired from these natural designs of ECM, nanotopography-guided approaches have been increasingly investigated for the last several decades. Results demonstrate that the nanotopography itself can activate tissue-specific function in vitro as well as promote tissue regeneration in vivo upon transplantation. In this review, we provide an extensive analysis of recent efforts to mimic functional nanostructures in vitro for improved tissue engineering and regeneration of injured and damaged tissues. We first characterize the role of various nanostructures in human tissues with respect to each tissue-specific function. Then, we describe various fabrication methods in terms of patterning principles and material characteristics. Finally, we summarize the applications of nanotopography to various tissues, which are classified into four types depending on their functions: protective, mechano-sensitive, electro-active, and shear stress-sensitive tissues. Some limitations and future challenges are briefly discussed at the end.