Expression of Collagen Types I and II on Articular Cartilage in a Rat Knee Contracture Model
Expression of Collagen Types I and II on Articular Cartilage in a Rat Knee Contracture Model
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DOI：
10.3109/03008200902859406
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Itoi, Eiji
中科院分区：
文献类型：
--
作者：
Hagiwara, Yoshihiro;Ando, Akira;Itoi, Eiji
我们研究的目的是阐明大鼠膝关节挛缩模型固定后 3 个特定区域（非接触区、过渡区、接触区）关节软骨上 I 型和 II 型胶原蛋白的表达模式。使用螺钉和刚性塑料板将成年雄性大鼠的单侧膝关节刚性固定在150度屈曲状态。假手术动物在股骨和胫骨上钻孔并插入螺钉，但不进行电镀。通过原位杂交（ISH）、免疫组织化学（IHC）和定量实时聚合酶链反应（qPCR）评估每个区域I型和II型胶原蛋白的表达模式。非接触区域 II 型胶原蛋白的表达因 ISH 而降低，但通过 IHC 检查时似乎没有变化。在过渡区和接触区，通过 ISH 检测，肥大软骨细胞中 II 型胶原蛋白的表达最初下降，然后升高，但通过 IHC 检测，II 型胶原蛋白的表达似乎下降。定量PCR显示接触区域II型胶原蛋白的表达减少。 I 型胶原蛋白的免疫染色在非接触区域和过渡区域增加。 I型和II型胶原表达的改变也可能影响固定后关节软骨的退变，并且三个区域的变化不同。
The purpose of our study was to clarify the expression patterns of collagen types I and II on articular cartilage after immobilization in a rat knee contracture model in 3 specific areas (noncontact area, transitional area, contact area). The unilateral knee joints of adult male rats were rigidly immobilized at 150 degrees of flexion using screws and a rigid plastic plate. Sham-operated animals had holes drilled in the femur and the tibia and screws inserted but were not plated. The expression patterns of collagen types I and II in each area were evaluated by in situ hybridization (ISH), immunohistochemistry (IHC), and quantitative real-time polymerase chain reaction (qPCR). The expression of collagen type II in the noncontact area was decreased by ISH but appeared unchanged when examined by IHC. In the transitional and contact areas, the expression of collagen type II was initially shown to have decreased and then increased at the hypertrophic chondrocytes by ISH but appeared decreased by IHC. Quantitative PCR revealed the decreased expression of type II collagen in the contact area. Immunostaining of collagen type I was increased at the noncontact area and transitional areas. Alterations of collagen types I and II expression may also affect the degeneration of articular cartilage after immobilization and the changes were different in the three areas.