Effect of altered masticatory function on [3H]-thymidine and [35S]-sulfate incorporation in the condylar cartilage of the rat.
Effect of altered masticatory function on [3H]-thymidine and [35S]-sulfate incorporation in the condylar cartilage of the rat.
复制标题
咀嚼功能改变对大鼠髁软骨中[3H]-胸苷和[35S]-硫酸盐掺入的影响。
DOI：
10.1159/000146501
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hinton,RJ
中科院分区：
文献类型：
--
作者：
Hinton,RJ
在活体条件下，对于关节压缩反作用力的变化，对髁状突软骨的反应研究甚少。为了减少或消除咀嚼或切开引起的咬合力，雄性断奶大鼠被喂以几乎不需要咀嚼的软饮食，和/或每隔一天修剪他们的门牙。与对照组相比，切割组的[~3H]-胸腺嘧啶核苷和[35S]-硫酸盐的掺入(DPM/微克DNA)显著降低，但在软饮食组中无显著差异。接受这两种治疗的动物也表现出明显低于对照组的纳入值，这表明在下颌关节切开进行负荷的重要性。这些数据证实了体外研究表明，压力可以影响有丝分裂活动和蛋白多糖的合成在髁突软骨。然而，在体内环境中，其他因素，无论是荷尔蒙的还是生物力学的，都可能是重要的。
The response of the condylar cartilage to alterations in compressive joint reaction forces in vivo has been little studied. In an attempt to reduce or eliminate the occlusal forces resulting from mastication or incision, male weanling rats were fed a soft diet requiring little chewing and/or had their incisors clipped every other day. Incorporation (dpm/µg DNA) of [3H]-thymidine and [35S]-sulfate was significantly decreased relative to controls in the incisorclipped group, but not in the soft-diet group. Animals having both treatments also exhibited significantly lower incorporation values than controls, suggesting the importance of incision for loading at the mandibular joint. These data corroborate in vitro studies which suggest that compressive forces can affect mitotic activity and synthesis of proteoglycans in the condylar cartilage. However, additional factors, both hormonal and biomechanical in nature, may be important in the in vivo environment.