Real-time fluorescence and deformability cytometry
Real-time fluorescence and deformability cytometry
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DOI：
10.1038/nmeth.4639
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发表时间：
2018-05-01
期刊：
影响因子：
48
通讯作者：
Guck, Jochen
中科院分区：
文献类型：
--
作者：
Rosendahl, Philipp;Plak, Katarzyna;Guck, Jochen
细胞机械表征的通量最近已经接近传统流式细胞仪的通量。然而，这种非常敏感、无标记的方法仍然缺乏分子标记的特异性。在这里，我们开发了一种在一台仪器（RT-FDC）中结合实时一维成像荧光和变形性细胞术的方法，从而开辟了许多新的研究途径。我们通过使用亚细胞荧光定位来识别有丝分裂细胞并在RNA干扰筛选中测试这些细胞中的机械变化来证明其实用性。
The throughput of cell mechanical characterization has recently approached that of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we developed an approach that combines real-time 1D-imaging fluorescence and deformability cytometry in one instrument (RT-FDC), thus opening many new research avenues. We demonstrated its utility by using subcellular fluorescence localization to identify mitotic cells and test for mechanical changes in those cells in an RNA interference screen.