Photosensitizer Encryption with Aggregation Enhanced Singlet Oxygen Production
Photosensitizer Encryption with Aggregation Enhanced Singlet Oxygen Production
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DOI：
10.1021/jacs.2c02596
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发表时间：
2022-06-22
影响因子：
15
通讯作者：
Payne, Daniel T.
中科院分区：
文献类型：
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作者：
Bloyet, Clarisse;Sciortino, Flavien;Payne, Daniel T.
在水中产生单线态氧（O-1（2））的发色团对于开发使用光动力疗法（PDT）的非侵入性疾病治疗至关重要。报道了一种制备稳定的O-1（2）光敏剂胶体纳米颗粒的简便方法，该方法显示出聚集增强的O-1（2）在水中的生成，从而可用作PDT试剂。品红芳烃大环支架内的发色团加密使光敏剂与聚集诱导的失活途径隔离，使得发色团密度比典型的O-1（2）生成纳米颗粒更高。观察到聚集增强了水中O-1（2）的生成，并且分子结构的变化允许调节纳米颗粒的物理性质，这最终影响O-1（2）的生成。使用共聚焦荧光显微镜用HeLa细胞证明了颗粒的体外活性和穿过细胞膜进入细胞质的能力。光敏剂加密刚性大环，如品红芳烃，为生产生物相容性纳米结构的应用提供了新的前景，涉及O-1（2）代。
Chromophores that generate singlet oxygen (O-1(2)) in water are essential to developing noninvasive disease treatments using photodynamic therapy (PDT). A facile approach for formation of stable colloidal nanoparticles of O-1(2) photosensitizers, which exhibit aggregation enhanced O-1(2) generation in water toward applications as PDT agents, is reported. Chromophore encryption within a fuchsonarene macrocyclic scaffold insulates the photosensitizer from aggregation induced deactivation pathways, enabling a higher chromophore density than typical O-1(2) generating nanoparticles. Aggregation enhanced O-1(2) generation in water is observed, and variation in molecular structure allows for regulation of the physical properties of the nanoparticles which ultimately affects the O-1(2) generation. In vitro activity and the ability of the particles to pass through the cell membrane into the cytoplasm is demonstrated using confocal fluorescence microscopy with HeLa cells. Photosensitizer encryption in rigid macrocycles, such as fuchsonarenes, offers new prospects for the production of biocompatible nanoarchitectures for applications involving O-1(2) generation.