Colonization-induced host-gut microbial metabolic interaction.
Colonization-induced host-gut microbial metabolic interaction.
复制标题
定植引起的宿主微生物代谢相互作用。
DOI：
10.1128/mbio.00271-10
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发表时间：
2011
期刊：
影响因子：
6.4
通讯作者：
Nicholson JK
中科院分区：
文献类型：
--
作者：
Claus SP;Ellero SL;Berger B;Krause L;Bruttin A;Molina J;Paris A;Want EJ;de Waziers I;Cloarec O;Richards SE;Wang Y;Dumas ME;Ross A;Rezzi S;Kochhar S;Van Bladeren P;Lindon JC;Holmes E;Nicholson JK
肠道微生物群增强了宿主加工营养和药物的代谢能力，并在各种器官系统中调节了多个途径的活性。使用高分辨率1H核磁共振（NMR）光谱的典型环境微生物背景，分析尿液，血浆，肝脏，肾脏和结肠（5次）点的代谢谱。触发肝触发合成的增加。包括脂肪损失的必不可少的调节剂，包括主要的药物 - 代谢酶的活性（CYP3A11和CYP2C29），这些代谢物也显着刺激了肝脏代谢的统计模型。 16s rRNA基因焦磷酸测序揭示了甲状腺细菌科的牢固关联与肝甘油三酸酯，葡萄糖和糖原水平以及异种生物的代谢。
肠道细菌与人类中的各种必不可少的生物学功能有关，例如，血压的调节，肠道微生物定植在出生后与其他关键过程（例如免疫和认知发展）同行。宿主代谢及其象征意义之间的双向相互作用。肝脂质代谢和肝脏代谢。改变宿主的微生物群体关系的策略，反过来可以有益地调节宿主代谢。
The gut microbiota enhances the host’s metabolic capacity for processing nutrients and drugs and modulate the activities of multiple pathways in a variety of organ systems. We have probed the systemic metabolic adaptation to gut colonization for 20 days following exposure of axenic mice (n = 35) to a typical environmental microbial background using high-resolution 1H nuclear magnetic resonance (NMR) spectroscopy to analyze urine, plasma, liver, kidney, and colon (5 time points) metabolic profiles. Acquisition of the gut microbiota was associated with rapid increase in body weight (4%) over the first 5 days of colonization with parallel changes in multiple pathways in all compartments analyzed. The colonization process stimulated glycogenesis in the liver prior to triggering increases in hepatic triglyceride synthesis. These changes were associated with modifications of hepatic Cyp8b1 expression and the subsequent alteration of bile acid metabolites, including taurocholate and tauromuricholate, which are essential regulators of lipid absorption. Expression and activity of major drug-metabolizing enzymes (Cyp3a11 and Cyp2c29) were also significantly stimulated. Remarkably, statistical modeling of the interactions between hepatic metabolic profiles and microbial composition analyzed by 16S rRNA gene pyrosequencing revealed strong associations of the Coriobacteriaceae family with both the hepatic triglyceride, glucose, and glycogen levels and the metabolism of xenobiotics. These data demonstrate the importance of microbial activity in metabolic phenotype development, indicating that microbiota manipulation is a useful tool for beneficially modulating xenobiotic metabolism and pharmacokinetics in personalized health care. Gut bacteria have been associated with various essential biological functions in humans such as energy harvest and regulation of blood pressure. Furthermore, gut microbial colonization occurs after birth in parallel with other critical processes such as immune and cognitive development. Thus, it is essential to understand the bidirectional interaction between the host metabolism and its symbionts. Here, we describe the first evidence of an in vivo association between a family of bacteria and hepatic lipid metabolism. These results provide new insights into the fundamental mechanisms that regulate host-gut microbiota interactions and are thus of wide interest to microbiological, nutrition, metabolic, systems biology, and pharmaceutical research communities. This work will also contribute to developing novel strategies in the alteration of host-gut microbiota relationships which can in turn beneficially modulate the host metabolism.