The complete genome of klassevirus - a novel picornavirus in pediatric stool
The complete genome of klassevirus - a novel picornavirus in pediatric stool
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DOI：
10.1186/1743-422x-6-82
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发表时间：
2009-06-18
期刊：
影响因子：
4.8
通讯作者：
DeRisi, Joseph L.
中科院分区：
文献类型：
--
作者：
Greninger, Alexander L.;Runckel, Charles;DeRisi, Joseph L.
背景：全球每年有 200 万儿童因腹泻而死亡，但约 30-50% 的病例未发现病因。小核糖核酸病毒属，如肠道病毒、曲布病毒、冠状病毒、副肠病毒、肝病毒、睾丸病毒和心脏病毒都已在人类和动物腹泻中发现。现代技术，尤其是深度测序，可以对粪便等临床样本进行快速、高通量筛查，以寻找与人类疾病相关的新传染源。结果：对先前发现已知腹泻病毒呈阴性的 141 份儿科胃肠炎样本进行了焦磷酸测序。从总共 937,935 个序列读数中，组装了与爱知病毒有远缘关系的 849 个读数，并发现它们包含新型小核糖核酸病毒基因组的 75%。随后克隆了完整的基因组，发现与爱知病毒具有 52.3% 的核苷酸配对同一性和 38.9% 的氨基酸同一性。低水平的序列同一性表明存在一种新的小核糖核酸病毒属，我们将其命名为克拉斯病毒。对来自有症状和无症状个体的 751 份粪便样本进行盲式筛查，结果发现第二例克拉斯病毒感染阳性，随后发现该病例来自指示病例 11 个月大的双胞胎。 结论：我们报告在来自北加州的两名婴儿的粪便中发现了人类克拉斯病毒 1，它是一种新型小核糖核酸病毒属的成员。需要进一步的表征和流行病学研究来确定类病毒是否是人类感染的重要原因。
Background: Diarrhea kills 2 million children worldwide each year, yet an etiological agent is not found in approximately 30-50% of cases. Picornaviral genera such as enterovirus, kobuvirus, cosavirus, parechovirus, hepatovirus, teschovirus, and cardiovirus have all been found in human and animal diarrhea. Modern technologies, especially deep sequencing, allow rapid, high-throughput screening of clinical samples such as stool for new infectious agents associated with human disease.Results: A pool of 141 pediatric gastroenteritis samples that were previously found to be negative for known diarrheal viruses was subjected to pyrosequencing. From a total of 937,935 sequence reads, a collection of 849 reads distantly related to Aichi virus were assembled and found to comprise 75% of a novel picornavirus genome. The complete genome was subsequently cloned and found to share 52.3% nucleotide pairwise identity and 38.9% amino acid identity to Aichi virus. The low level of sequence identity suggests a novel picornavirus genus which we have designated klassevirus. Blinded screening of 751 stool specimens from both symptomatic and asymptomatic individuals revealed a second positive case of klassevirus infection, which was subsequently found to be from the index case's 11-month old twin.Conclusion: We report the discovery of human klassevirus 1, a member of a novel picornavirus genus, in stool from two infants from Northern California. Further characterization and epidemiological studies will be required to establish whether klasseviruses are significant causes of human infection.