Dye-Sensitized Downconversion Nanoprobes with Emission Beyond 1500 nm for Ratiometric Visualization of Cancer Redox State
Dye-Sensitized Downconversion Nanoprobes with Emission Beyond 1500 nm for Ratiometric Visualization of Cancer Redox State
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发射波长超过 1500 nm 的染料敏化下转换纳米探针，用于癌症氧化还原状态的比例可视化
DOI：
10.1002/adfm.202009942
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Song Jibin
中科院分区：
文献类型：
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作者：
Wang Chenlu;Lin Hongxin;Ge Xiaoguang;Mu Jing;Su Lichao;Zhang Xuan;Niu Meng;Yang Huanghao;Song Jibin
体内谷胱甘肽(GSH)的检测对于准确地描绘细胞的氧化还原状态和实时显示体内的生理和病理状态是必不可少的。然而，由于组织的自发荧光背景和灾难性的光子散射，传统的近红外I区(NIR-I，650-900 nm)的荧光成像很难定量显示体内的GSH。在此，我们开发了一种由4-硝基苯酚-Cy7(NPH)共轭的稀土下转换纳米颗粒(DCNP@NPH-PEG)组成的NIR-IIb(1500-1700 nm)纳米探针，用于体内GSH的比率成像。在GSH存在下，NPH通过非辐射能量转移(NRET)效应增强了DCNP在808 nm处的FL发射，从而增强了DCNP在1550 nm处的FL信号，而在980 nm激光(F1550,980Ex)激发下的DCNP在1550 nm处的荧光是稳定的，因为没有发生NRET。比值F1550、980Ex/F1550、808Ex值与GSH浓度在0-24 mM范围内呈线性关系，检出限为0.3 mM。NIR-IIb纳米探针在体内对皮下肿瘤和原位结肠肿瘤中的GSH具有良好的检测和成像性能，具有较高的准确性和分辨率。基于激活Fert效应的比率计量型NIR-II FL纳米探针的设计策略为开发用于体内精确生物传感的NIR-II纳米探针提供了可靠的工具。
Detection of glutathione (GSH) in the body is essential to accurately map the redox state of cells and real‐time visualization of physiological and pathological conditions in vivo. However, traditional fluorescence (FL) imaging in the near‐infrared I region (NIR‐I, 650–900 nm) is difficult to quantitively visualize GSH in vivo due to the tissue autofluorescence background and disastrous photon scattering. Herein, a NIR‐IIb(1500–1700 nm) nanoprobe consisting of 4‐nitrophenol‐Cy7 (NPh) conjugated lanthanide‐based downconversion nanoparticles (DCNP@NPh‐PEG) is developed for in vivo ratiometric imaging of GSH. In the presence of GSH, NPh shows responsively enhanced FL emission at 808 nm, thus enhancing FL signal at 1550 nm of DCNPs excited by 808 nm (F1550, 808Ex) through non‐radiative energy transfer (NRET) effect, while the fluorescence of DCNP at 1550 nm excited by 980 nm laser (F1550, 980Ex) is stable because no NRET occurred. The ratiometric F1550, 980Ex/F1550, 808Exvalue exhibits a linearship with GSH concentration ranged from 0–24 mmwith detection limit of 0.3 mm. The NIR‐IIbnanoprobe has excellent performance in detecting and imaging GSH in both subcutaneous tumor and orthotopic colon tumor in vivo with high accuracy and resolution. The design strategy of the ratiometric NIR‐II FL nanoprobe based on the activated FERT effect provides a reliable tool for the development of NIR‐II nanoprobes for accurate biosensing in vivo.