Changes in gene expression following high-temperature adaptation in experimentally evolved populations of E. coli.
Changes in gene expression following high-temperature adaptation in experimentally evolved populations of E. coli.
复制标题
实验进化的大肠杆菌群体高温适应后基因表达的变化。
DOI：
10.1086/430035
复制
发表时间：
2005
期刊：
影响因子：
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通讯作者：
Long,AnthonyD
中科院分区：
文献类型：
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作者：
Riehle,MichelleM;Bennett,AlbertF;Long,AnthonyD
转录谱分析（RNA丰度的定量分析）可以提供一个 伴随生物体的基因表达变化的全基因组图片 适应新的环境。在这里，我们使用DNA微阵列来表征 三个复制系中转录本丰度的全基因组变化 细菌E.共育了2,000代 高温胁迫（41.5°C）。在这些细胞系中，12%的基因 在高温适应过程中显著改变表达; 这些变化中的大多数（55%）小于两倍的增加或减少。 39个基因，是随机预期数量的4倍 跨系表现出中度或高度重复的表达变化。 在先验定义的功能类别内的表达变化显示出均匀的 比单个基因的复制水平更高。表达变化 表型定义的胁迫基因和适应功能类别 在进化适应高温中起着重要作用。
Transcription profiling (quantitative analysis of RNA abundance) can provide a genome‐wide picture of gene expression changes that accompany organismal adaptation to a new environment. Here, we used DNA microarrays to characterize genome‐wide changes in transcript abundance in three replicate lines of the bacteriumE. coligrown for 2,000 generations at a stressful high temperature (41.5°C). Across these lines, 12% of genes significantly changed expression during high‐temperature adaptation; the majority of these changes (55%) were less than twofold increments or decrements. Thirty‐nine genes, four times the number expected by chance alone, exhibited moderately or highly replicated expression changes across lines. Expression changes within a priori defined functional categories showed an even greater level of replication than did individual genes. Expression changes in the phenotypically defined stress genes and adaptation functional categories were important in evolutionary adaptation to high temperature.