Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration.
Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration.
复制标题
DOI：
10.3390/ma10010033
复制
发表时间：
2017-01-03
期刊：
影响因子：
--
通讯作者：
Saito N
中科院分区：
文献类型：
--
作者：
Tanaka M;Haniu H;Kamanaka T;Takizawa T;Sobajima A;Yoshida K;Aoki K;Okamoto M;Kato H;Saito N
单向多孔羟基磷灰石HAp（UDPHAp）是一种轴向具有连续连通孔结构的支架。我们评估并比较了UDPHAp作为三维（3D）骨组织工程支架与相互连接的钙多孔HAp陶瓷（IP-CHA）的能力。为了实现这一点，我们在体外评估的抗压强度，控制rhBMP-2的释放行为，粘附细胞形态，细胞粘附方式，和细胞附着的UDPHAp。作为进一步的体内实验，将UDPHAp和IP-CHA与rhBMP-2一起移植到小鼠颅骨缺损中，以评价其成骨能力。结果表明，UDPHAp的最大压缩强度为7.89 ± 1.23 MPa，高于IP-CHA的最大压缩强度（1.92 ± 0.53 MPa）（p = 0.0039）。然而，断裂能相似（8.99 ± 2.72 vs. 13.95 ± 5.69 mJ，p = 0.055）。UDPHAp在体内释放rhBMP-2更缓慢。UDPHAp上的细胞紧紧地粘附在表面上，这些细胞已经深深地生长到支架中。在第8天，与IP-CHA相比，观察到UDPHAp上的细胞数量显著增加（102，479 ± 34，391对32，372 ± 29，061个估计细胞/支架，p = 0.0495）。在小鼠颅骨缺损模型中，IP-CHA组和UDPHAp组的2x视野中新骨面积（成熟骨+松质骨）百分比分别为2.514% ± 1.224%和7.045% ± 2.055%，UDPHAp组的百分比显著更高（p = 0.0209）。在保持与IP-CHA相同的强度的同时，具有84%孔隙率的UDPHAp显示出高细胞数量、高细胞侵袭性和优异的骨形成。我们认为UDPHAp是一种可以应用于骨再生医学的优良材料。
The unidirectional porous hydroxyapatite HAp (UDPHAp) is a scaffold with continuous communicated pore structure in the axial direction. We evaluated and compared the ability of the UDPHAp as a three-dimensional (3D) bone tissue engineering scaffold to the interconnected calcium porous HAp ceramic (IP-CHA). To achieve this, we evaluated in vitro the compressive strength, controlled rhBMP-2 release behavior, adherent cell morphology, cell adhesion manner, and cell attachment of UDPHAp. As a further in vivo experiment, UDPHAp and IP-CHA with rhBMP-2 were transplanted into mouse calvarial defects to evaluate their bone-forming ability. The Results demonstrated that the maximum compressive strengths of the UDPHAp was 7.89 ± 1.23 MPa and higher than that of IP-CHA (1.92 ± 0.53 MPa) (p = 0.0039). However, the breaking energies were similar (8.99 ± 2.72 vs. 13.95 ± 5.69 mJ, p = 0.055). The UDPHAp released rhBMP-2 more gradually in vivo. Cells on the UDPHAp adhered tightly to the surface, which had grown deeply into the scaffolds. A significant increase in cell number on the UDPHAp was observed compared to the IP-CHA on day 8 (102,479 ± 34,391 vs. 32,372 ± 29,061 estimated cells per scaffold, p = 0.0495). In a mouse calvarial defect model, the percentages of new bone area (mature bone + trabecular bone) in the 2x field were 2.514% ± 1.224% for the IP-CHA group and 7.045% ± 2.055% for the UDPHAp group, and the percentage was significantly higher in the UDPHAp group (p = 0.0209). While maintaining the same strength as the IP-CHA, the UDPHAp with 84% porosity showed a high cell number, high cell invasiveness, and excellent bone formation. We believe the UDPHAp is an excellent material that can be applied to bone regenerative medicine.