What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo?
What can microdialysis tell us about the temporal and spatial generation of cytokines in allergen-induced responses in human skin in vivo?
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DOI：
10.1038/sj.jid.5700930
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发表时间：
2007-12-01
影响因子：
6.5
通讯作者：
Church, Martin K.
中科院分区：
文献类型：
--
作者：
Clough, Geraldine F.;Jackson, Claire L.;Church, Martin K.
This study examined the suitability of microdialysis to assess the time course of cytokine generation from discrete sites within the skin following intradermal injection of allergen. Cytokines were recovered using two microdialysis probes, one close to the point of allergen injection and the other 1cm away but within the area of the late-phase induration. Skin biopsies taken at both sites were stained immunocytochemically to investigate possible relationships between cytokine generation, expression of adhesion molecules, and recruitment of neutrophils and eosinophils during the late-phase allergic response. The cytokine response to probe insertion was assessed using a single probe in the opposite arm ( control). At baseline, microdialysate contained low levels of IL-1 alpha, IL-5, IL-8, IL-12, GM-CSF, and TNF alpha(n=27-33). At control sites, this was followed by increases in IL-6 and IL-8 at 3 and 6 hours. Allergen increased TNF alpha levels in 3/11 individuals within 30 minutes at the injection site. Levels of IL-6 and IL-8 rose rapidly and were significantly greater (P
