Correlation of hydrogen exchange behaviour and thermal stability of lysozyme.
Correlation of hydrogen exchange behaviour and thermal stability of lysozyme.
复制标题
溶菌酶氢交换行为与热稳定性的相关性。
DOI：
10.1016/s0022-2836(83)80309-x
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发表时间：
1983
影响因子：
5.6
通讯作者：
F. Poulsen
中科院分区：
文献类型：
--
作者：
M. Delepierre;C. Dobson;S. Selvarajah;R. Wedin;F. Poulsen
本文用~ 1H核磁共振法测定了溶菌酶色氨酸残基上吲哚NH氢的溶剂交换速率随温度的变化。结果已被解释在一个低活化能的过程，这是不依赖于蛋白质的热稳定性，和一个较高的活化能的过程，这是直接与热稳定性。这些意见的蛋白质的动力学的理解的意义进行了讨论。
The solvent exchange rates of individual indole NH hydrogens of tryptophan residues of lysozyme have been measured, by using1H nuclear magnetic resonance spectroscopy, as a function of temperature in the presence of urea and following chemical modification. The results have been interpreted in terms of a low activation energy process which is not dependent on the thermal stability of the protein, and a higher activation energy process that is directly correlated with the thermal stability. The significance of these observations for an understanding of the dynamics of the protein is discussed.
溶菌酶中单个色氨酸吲哚 NH 氢的核磁共振研究蛋白质中氢交换的机制。
影响因子：
2.9
作者：
Wedin,RE;Delepierre,M;Dobson,CM;Poulsen,FM
通讯作者：
Poulsen,FM