Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis
Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis
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DOI：
10.1038/emboj.2009.4
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发表时间：
2009-03-04
期刊：
影响因子：
11.4
通讯作者：
Ulm, Roman
中科院分区：
文献类型：
--
作者：
Favory, Jean-Jacques;Stec, Agnieszka;Ulm, Roman
太阳辐射的紫外线b （UV-B）部分作为一种环境信号，植物已经进化出特异性和敏感的UV-B感知系统。UV- b特异性UV反应位点8 （UVR8）和多功能E3泛素连接酶CONSTITUTIVELY PHOTOMORPHOGENIC 1 （COP1）是UV- b反应的关键调控因子。我们在这里发现，UVR8缺失突变体缺乏UV-B诱导的光形态发生，对UV-B胁迫敏感，而UVR8过表达导致UV-B光形态发生、驯化和对UV-B胁迫的耐受性增强。通过使用太阳模拟器，我们在生理水平上提供了由UV-B特异性光调节途径介导的UV-B驯化确实是在阳光下生存所必需的证据。在分子水平上，我们证明了野生型而不是突变型UVR8和COP1蛋白在植物中以依赖uvb的快速方式直接相互作用。这些数据共同表明，细胞核中COP1和UVR8的UV-B特异性相互作用是信号传导的早期步骤，并负责植物对UV-B的协调响应，确保在自然环境中适应和保护UV-B。
The ultraviolet-B (UV-B) portion of the solar radiation functions as an environmental signal for which plants have evolved specific and sensitive UV-B perception systems. The UV-B-specific UV RESPONSE LOCUS 8 (UVR8) and the multifunctional E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) are key regulators of the UV-B response. We show here that uvr8-null mutants are deficient in UV-B-induced photomorphogenesis and hypersensitive to UV-B stress, whereas overexpression of UVR8 results in enhanced UV-B photomorphogenesis, acclimation and tolerance to UV-B stress. By using sun simulators, we provide evidence at the physiological level that UV-B acclimation mediated by the UV-B-specific photoregulatory pathway is indeed required for survival in sunlight. At the molecular level, we demonstrate that the wild type but not the mutant UVR8 and COP1 proteins directly interact in a UVB-dependent, rapid manner in planta. These data collectively suggest that UV-B-specific interaction of COP1 and UVR8 in the nucleus is a very early step in signalling and responsible for the plant's coordinated response to UV-B ensuring UV-B acclimation and protection in the natural environment.