Evidence that ecdysteroids and methyl farnesoate control allometric growth and differentiation in a crustacean
Evidence that ecdysteroids and methyl farnesoate control allometric growth and differentiation in a crustacean
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DOI：
10.1016/s0965-1748(01)00104-7
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发表时间：
2002-02-01
影响因子：
3.8
通讯作者：
Baclaski, B
中科院分区：
文献类型：
--
作者：
Laufer, H;Ahl, J;Baclaski, B
身体各部分的异速生长或不成比例的生长是形态发生中的一个基本问题。雄性蜘蛛蟹，Libinia embryata，有几种形式或形态型。在终末蜕皮期间，前足不成比例地扩大，超过甲壳长度多达35%。尽管短爪雄性是生殖的，但大爪雄性成为主要的生殖动物。我们刺激倒数第二个阶段的男性蜕皮眼柄消融，去除蜕皮抑制激素（MIH）和下颌器官抑制激素（MIOH），并测定蜕皮激素的放射免疫分析和甲基法尼酸（MF）的血淋巴中的高效液相色谱法（HPLC）使用内标。眼柄消融加速蜕皮和蜕皮激素增加峰值在150毫微克/毫升前蜕皮。在对照组动物中，蜕皮激素在蜕皮前3天达到90 ng/ml的峰值，MF保持低于0.5 ng/ml。它们变成了长着异速生长的大爪子的大雄性。相比之下，消融的，增加MF（1至1.5纳克/毫升），增加甲壳大小，但保留较短的非异速生长爪，长度短于甲壳。结果与我们用MF施用进行的实验一致（Abdu et al.，生物公牛，伍兹霍尔，MA 195（1998）112; Laufer等人，内分泌综合征111（1998）113; Laufer等人，在：IV Symp.《中美洲的水生生物：聚焦于虾和罗非鱼》，（1997年a），第161页; Laufer等人，倒过来Reprod. Dev. 31（1997 b）63），这导致解释蜕皮甾类和低MF浓度促进异速生长，而具有相对较高MF浓度的蜕皮激素抑制异速生长。这些结果表明并支持MF和蜕皮激素决定了甲壳动物异速生长中形态建成的控制的结论。(C)2002爱思唯尔科技有限公司版权所有。
Allometric, or disproportionate, growth of body parts is a basic problem in morphogenesis. Male spider crabs, Libinia emarginata, have several forms or morphotypes. During the terminal molt, the propodus enlarges disproportionately, exceeding the carapace length by as much as 35%. Even though shorter clawed males are reproductive, the large-clawed males become primary reproductives. We stimulated penultimate stage males to molt by eyestalk ablation, which removes molt inhibiting hormone (MIH) and mandibular organ inhibiting hormone (MIOH), and measured ecdysones by radioimmunoassay and methyl farnesoate (MF) in hemolymph by high-performance liquid chromatography (HPLC) using an internal standard. Eyestalk ablation accelerated molting and increased ecdysteroids to peak at 150 ng/ml before the molt. In control animals the ecdysteroids peaked at 90 ng/ml 3 days before the molt, with MF remaining less than 0.5 ng/ml. These became large males with large allometric claws. In contrast, the ablated ones, with increased MF (1 to 1.5 ng/ml), increased carapace size, but retained shorter non-allometric claws, with length shorter than the carapace. The results are consistent with experiments that we have performed with MF administration (Abdu et al., Biol. Bull., Woods Hole, MA 195 (1998) 112; Laufer et al., Gen. Comp. Endocrinol. 111 (1998) 113; Laufer et al., in: IV Symp. Aquaculture in Central America: Focusing on Shrimp and Tilapia, (1997a), p. 161; Laufer et al., Invert. Reprod. Dev. 31 (1997b) 63) which led to the interpretation that ecdysteroids and low MF concentrations promote allometric growth, while ecdysones with relatively higher concentrations of MF inhibited allometric growth. These results indicate and support the conclusion that MF and ecdysteroids determine the control of morphogenesis in allometric growth of Crustacea. (C) 2002 Elsevier Science Ltd. All rights reserved.