A novel Pax-6 binding site in rodent B1 repetitive elements: coevolution between developmental regulation and repeated elements?
A novel Pax-6 binding site in rodent B1 repetitive elements: coevolution between developmental regulation and repeated elements?
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啮齿动物 B1 重复元件中的新 Pax-6 结合位点：发育调节和重复元件之间的协同进化？
DOI：
10.1016/s0378-1119(00)00019-6
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发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Saunders,GF
中科院分区：
文献类型：
--
作者：
Zhou,Y;Zheng,JB;Gu,X;Li,W;Saunders,GF
Pax-6 encodes a transcription factor that is important in the development of eye and CNS. Identification of Pax-6 target genes is crucial for understanding the gene regulatory network in these developmental processes. Using an in-vitro approach of cyclic amplification of the protein binding sequences (CAPBS), we isolated a PAX6 binding sequence from a human single-copy (sc) DNA library. Characterization of this PAX6 binding sequence revealed a 15bp region (hGCα1BLs5) that is sufficient for PAX6 specific binding. From a homology search in the GenBank, we found that an hGCα1BLs5-like Pax-6 binding site exists in 21 genes (16 from rodent), 15 of which were shown to be able to bind Pax-6 in vitro. Interestingly, some of these sites occur in B1 repetitive elements. Although hGCα1BLs5 is highly similar to a region in B1 repetitive elements, PAX6 does not bind to the consensus sequence in B1. However, a single-step mutation in some B1 elements can lead to a gain of function for PAX6 binding. This experimental evidence and phylogenetic analysis raise an interesting speculation for the coevolution between PAX6 regulation and repeat elements. Since a (Pax-6-binding) null B1 element can be re-activated by even a single-step mutation, it has the potential to recruit gene targets for Pax-6 if it is inserted into the regulatory region, and therefore may play a role for evolutionary modification of Pax-6 regulation.
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影响因子：
14.9
作者：
Y. Quentin
通讯作者：
Y. Quentin
核前 mRNA 的长双链序列 (dsRNA-B) 由几类高度丰富的序列组成：来自 DNA 克隆实验的证据。
影响因子：
14.9
作者：
D. A. Kramerov;A. A. Grigoryan;A. Ryskov;G. Georgiev
通讯作者：
G. Georgiev
人类基因组中重复 DNA 序列的群体。
影响因子：
5.6
作者：
G. Saunders;S. Shirakawa;P. Saunders;F. Arrighi;T. Hsu
通讯作者：
T. Hsu
影响因子：
4
作者：
NOLL, M
通讯作者：
NOLL, M
影响因子：
3.5
作者：
Tang, HK;Chao, LY;Saunders, GF
通讯作者：
Saunders, GF