Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8
Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8
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DOI：
10.1002/mrd.23682
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发表时间：
2023-03
影响因子：
2.5
通讯作者：
Yingjie Wang;Lei Zhang;Ruihong Kong;Cai Hu;Zongyi Zhao;Yuhui Wu;Qisheng Zuo;Bichun Li;Ya‐ni Zhang
中科院分区：
文献类型：
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作者：
Yingjie Wang;Lei Zhang;Ruihong Kong;Cai Hu;Zongyi Zhao;Yuhui Wu;Qisheng Zuo;Bichun Li;Ya‐ni Zhang
减数分裂是精子发生的关键步骤，受多种因素的影响。目前的研究表明，长非编码RNA(Long Non Coding RNAs，LncRNAs)是调节减数分裂的潜在因子，其调控机制备受关注。然而，目前对其在公鸡精子发生中的调控机制研究较少。在这里，我们发现参与减数分裂和精子发生的lncRNA(lncRNA-IMS)参与了gga-miR-31-5p对Stra8的调节，并阻碍了gga-miR-31-5p对Stra8的抑制。获得和丧失功能的实验证明，lncRNA-IMS参与了减数分裂和精子发生。此外，我们还预测并确定了lncRNA-IMS的核心启动子区域。对转录因子的预测、结合位点的缺失/过表达、Jun基因的敲除/过表达以及双荧光素酶报告基因的分析证实，Jun正向激活了lncRNA-IMS的转录。我们的发现进一步丰富了男性减数分裂过程中的Tf-lncRNA-miRNA-mRNA调控网络，为研究鸡精原干细胞减数分裂和精子发生的分子机制提供了新的思路。
Meiosis, a key step in spermatogenesis, is affected by many factors. Current studies have shown that long noncoding RNAs (lncRNAs) are potential factors regulating meiosis, and their regulatory mechanisms have received much attention. However, little research has been done on its regulatory mechanism in the spermatogenesis of roosters. Here, we found that lncRNA involved in meiosis and spermatogenesis (lncRNA‐IMS) was involved in the regulation of Stra8 by gga‐miR‐31‐5p and hindered the inhibition of Stra8 by gga‐miR‐31‐5p. The acquisition and loss of function experiments demonstrated that lncRNA‐IMS was involved in meiosis and spermatogenesis. In addition, we predicted and determined the core promoter region of lncRNA‐IMS. Prediction of transcription factors, deletion/overexpression of binding sites, knockdown/overexpression of Jun, and dual‐luciferase reporter analysis confirmed that Jun positively activated transcription of lncRNA‐IMS. Our findings further enrich the TF‐lncRNA‐miRNA‐mRNA regulatory network during male meiosis and provide new ideas for studying the molecular mechanism of meiosis and spermatogenesis in chicken spermatogonial stem cells.