Quantification of cholesterol nucleation promoting activity in human gallbladder bile.
Quantification of cholesterol nucleation promoting activity in human gallbladder bile.
复制标题
人胆囊胆汁中胆固醇成核促进活性的定量。
DOI：
10.1016/0009-8981(87)90174-4
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
G. Tytgat
中科院分区：
文献类型：
--
作者：
J. Drapers;A. Groen;J. P. Stout;C. Noordam;F. Hoek;P. Jansen;G. Tytgat
我们已经开发了一种简单的方法来定量胆汁中胆固醇成核促进活性。该方法利用胆固醇结石患者的胆囊胆汁含有有效的成核促进活性的事实。对胆囊胆汁样本进行系列稀释，常规稀释范围为1/25至1/6400。将稀释的样品与过饱和模型胆汁混合，并测定混合物的成核时间（NT）。导致NT显著缩短的最大稀释度称为成核促进活性滴度（NPAT）。该测定与胆汁样品的原始脂质含量无关。对14例胆固醇结石患者和9例正常人的胆囊胆汁进行了NPAT测定。在所有样品中发现促进活性。在结石患者的样品中，与没有胆固醇结石的患者相比，NPAT显著升高（p= 0.01）。结果提示，胆固醇饱和指数和胆固醇成核促进因子的活性是胆固醇结石病发病机制中最重要的因素。NPAT的评估允许区分胆固醇饱和指数正常的患者组，这些患者由于NPAT高而有胆结石形成的风险。
We have developed a simple method to quantitate cholesterol nucleation promoting activity in bile. The method makes use of the fact that gallbladder bile of cholesterol gallstone patients contains potent nucleation promoting activity. Gallbladder bile samples were serially diluted, routinely from 1/25 to 1/6400. The diluted samples were mixed with a supersaturated model bile and the nucleation time (NT) of the mixtures was determined. The greatest dilution that resulted in a significant shortening of the NT was called the nucleation promoting activity titre (NPAT). The determination is independent of the original lipid content of the bile sample. The NPAT was measured in 14 gallbladder bile samples derived from patients with cholesterol gallstones and 9 controls. In all samples promoting activity was found. In the samples from the stone patients the NPAT was significantly elevated as compared to the patients without cholesterol stones (p= 0.01). Our results suggest that the cholesterol saturation index and the activity of cholesterol nucleation promoting factors are the most important factors in the pathogenesis of cholesterol gallstone disease. Assessment of the NPAT allows the differentiation of groups of patients with a normal cholesterol saturation index who are at risk for gallstone formation due to a high NPAT.
胆汁蛋白质。
DOI：
10.1172/jci111204
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Holzbach,RT;Kibe,A;Thiel,E;Howell,JH;Marsh,M;Hermann,RE
通讯作者：
Hermann,RE
影响因子：
6.5
作者：
Gurantz,D;Laker,MF;Hofmann,AF
通讯作者：
Hofmann,AF