IDENTIFICATION OF HUMAN AND BOVINE ROTAVIRUS SEROTYPES BY POLYMERASE CHAIN-REACTION
IDENTIFICATION OF HUMAN AND BOVINE ROTAVIRUS SEROTYPES BY POLYMERASE CHAIN-REACTION
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DOI：
10.1017/s0950268800050263
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发表时间：
1992-10-01
影响因子：
4.2
通讯作者：
URASAWA, S
中科院分区：
文献类型：
--
作者：
TANIGUCHI, K;WAKASUGI, F;URASAWA, S
应用聚合酶链反应（PCR）鉴定人轮状病毒和牛轮状病毒血清型。在对115份人粪便轮状病毒样本的鉴定中，PCR结果与使用血清型特异性单克隆抗体的酶联免疫吸附试验（ELISA）的血清分型结果非常吻合。此外，PCR的灵敏度（93%）远高于ELISA（82.6%）。该方法也可用于牛轮状病毒血清型的鉴定。
The use of the polymerase chain reaction (PCR) for identifying serotypes of human and bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in stools, results with PCR showed excellent agreement with results of serotyping by an enzyme-linked immunosorbent assay (ELISA) using serotype-specific monoclonal antibodies. Furthermore, the PCR showed a much higher sensitivity (93%) than the ELISA test (82.6%). The PCR method could also be applied for identifying the serotype of bovine rotaviruses.