Identification of Rehmannia glutinosa L. NB-ARC family proteins and their typical changes under consecutive monoculture stress
Identification of Rehmannia glutinosa L. NB-ARC family proteins and their typical changes under consecutive monoculture stress
复制标题
地黄NB-ARC家族蛋白的鉴定及其在连续单一胁迫下的典型变化
DOI：
10.1007/s11738-018-2672-1
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发表时间：
2018-05-01
影响因子：
2.6
通讯作者：
Zhang, Zhongyi
中科院分区：
文献类型：
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作者：
Chen, Aiguo;Gu, Li;Zhang, Zhongyi
NB-ARC蛋白对效应物触发免疫至关重要，在植物健康生长的效应物识别和信号转导中发挥重要作用。然而，它们在地黄中的主要蛋白质特性、功能和作用尚不完全清楚。在此，我们从粘地黄菌的蛋白序列集和转录组中鉴定出45个NB-ARC蛋白序列。CC型为主要类型，占84.44%。最保守的基序是一个288 aa的adp结合序列。该基序属于抗病蛋白。36个表达的NB-ARC基因的差异表达表明，NB-ARC基因在种植后30天很少表达，在种植后60天左右频繁表达。为了进一步了解NB-ARC在地黄再植中的功能，利用qRT-PCR技术对连作单作问题形成过程中不同胁迫状态下NB-ARC结构域的编码基因进行了分析。结果表明，NB-ARC基因可能在地黄连作问题的形成中起作用。本研究首次在地黄中鉴定出NB-ARC基因，并揭示了其在地黄连续单作问题中的作用。这些发现提供了对连续单一栽培问题形成机制的见解。
NB-ARC proteins are critical to effector-triggered immunity and play important roles in effector recognition and signal transduction in healthy plant growth. However, their primary protein traits, functions and roles remain incompletely understood in Rehmannia glutinosa. Here, we identified 45 NB-ARC protein sequences from the protein sequence sets and transcriptome of R. glutinosa. The CC type was the main one, accounting for 84.44% of these sequences. The most conserved motif was a 288 aa ADP-binding sequence. This motif belongs to the disease-resistance proteins. Differential expression of 36 expressed NB-ARC genes revealed that NB-ARC genes were rarely expressed 30 days after planting and were frequently expressed approximately 60 days after planting. To further understand the function of NB-ARC in replanted R. glutinosa, the genes encoding NB-ARC domains were profiled using qRT-PCR under the different stress states involved in the formation of consecutive monoculture problems. The results showed that NB-ARC genes might play a role in the formation of R. glutinosa consecutive monoculture problems. This study is the first to identify NB-ARC genes in R. glutinosa and to reveal their roles in consecutive monoculture problems in R. glutinosa. These findings provide insights into the mechanism of formation of consecutive monoculture problems.